TU Darmstadt / ULB / TUbiblio

Clathrin-Independent Endocytosis Contributes to Uptake of Glucose into BY-2 Protoplasts.

Bandmann, Vera and Homann, Ulrike (2012):
Clathrin-Independent Endocytosis Contributes to Uptake of Glucose into BY-2 Protoplasts.
In: The Plant journal : for cell and molecular biology, pp. 578-584, 70, (4), ISSN 1365-313X, [Article]

Abstract

In eukaryotic cells several pathways for the internalisation of plasma membrane proteins and extracellular cargo molecules exist. These endocytic pathways can be grouped into clathrin-dependent and clathrin-independent endocytosis. While the former one has been described to be involved in a variety of cellular processes in plants, the latter one has so far only been identified in animal and yeast cells. Here we show that internalisation of fluorescent glucose into BY-2 cells leads to accumulation of the sugar into compartments of the endocytic pathway. This endocytic uptake of glucose was not blocked by ikarugamycin (IKA), an inhibitor of clathrin-dependent endocytosis, suggesting a role for clathrin-independent endocytosis in glucose uptake. Investigations of fusion and fission of single vesicles by membrane capacitance measurements revealed a stimulation of endocytic activity by extracellular glucose. Glucose stimulated fission of vesicles was not affected by addition of IKA or block of clathrin coat formation by transient over-expression HUB1 (C-terminal part of the clathrin heavy chain). These data demonstrate that clathrin-independent endocytosis does exist in plant cells. This pathway may represent a common mechanism for the uptake of external nutrients. © 2011 The Authors. The Plant Journal© 2011 Blackwell Publishing Ltd.

Item Type: Article
Erschienen: 2012
Creators: Bandmann, Vera and Homann, Ulrike
Title: Clathrin-Independent Endocytosis Contributes to Uptake of Glucose into BY-2 Protoplasts.
Language: English
Abstract:

In eukaryotic cells several pathways for the internalisation of plasma membrane proteins and extracellular cargo molecules exist. These endocytic pathways can be grouped into clathrin-dependent and clathrin-independent endocytosis. While the former one has been described to be involved in a variety of cellular processes in plants, the latter one has so far only been identified in animal and yeast cells. Here we show that internalisation of fluorescent glucose into BY-2 cells leads to accumulation of the sugar into compartments of the endocytic pathway. This endocytic uptake of glucose was not blocked by ikarugamycin (IKA), an inhibitor of clathrin-dependent endocytosis, suggesting a role for clathrin-independent endocytosis in glucose uptake. Investigations of fusion and fission of single vesicles by membrane capacitance measurements revealed a stimulation of endocytic activity by extracellular glucose. Glucose stimulated fission of vesicles was not affected by addition of IKA or block of clathrin coat formation by transient over-expression HUB1 (C-terminal part of the clathrin heavy chain). These data demonstrate that clathrin-independent endocytosis does exist in plant cells. This pathway may represent a common mechanism for the uptake of external nutrients. © 2011 The Authors. The Plant Journal© 2011 Blackwell Publishing Ltd.

Journal or Publication Title: The Plant journal : for cell and molecular biology
Volume: 70
Number: 4
Divisions: 10 Department of Biology > Plant Cell Biology
?? fb10_botanik ??
10 Department of Biology
Date Deposited: 10 Jan 2012 08:26
Export:

Optionen (nur für Redakteure)

View Item View Item