Bandmann, Vera ; Homann, Ulrike (2012):
Clathrin-Independent Endocytosis Contributes to Uptake of Glucose into BY-2 Protoplasts.
In: The Plant journal : for cell and molecular biology, 70 (4), pp. 578-584. ISSN 1365-313X,
[Article]
Abstract
In eukaryotic cells several pathways for the internalisation of plasma membrane proteins and extracellular cargo molecules exist. These endocytic pathways can be grouped into clathrin-dependent and clathrin-independent endocytosis. While the former one has been described to be involved in a variety of cellular processes in plants, the latter one has so far only been identified in animal and yeast cells. Here we show that internalisation of fluorescent glucose into BY-2 cells leads to accumulation of the sugar into compartments of the endocytic pathway. This endocytic uptake of glucose was not blocked by ikarugamycin (IKA), an inhibitor of clathrin-dependent endocytosis, suggesting a role for clathrin-independent endocytosis in glucose uptake. Investigations of fusion and fission of single vesicles by membrane capacitance measurements revealed a stimulation of endocytic activity by extracellular glucose. Glucose stimulated fission of vesicles was not affected by addition of IKA or block of clathrin coat formation by transient over-expression HUB1 (C-terminal part of the clathrin heavy chain). These data demonstrate that clathrin-independent endocytosis does exist in plant cells. This pathway may represent a common mechanism for the uptake of external nutrients. © 2011 The Authors. The Plant Journal© 2011 Blackwell Publishing Ltd.
| Item Type: | Article |
|---|---|
| Erschienen: | 2012 |
| Creators: | Bandmann, Vera ; Homann, Ulrike |
| Title: | Clathrin-Independent Endocytosis Contributes to Uptake of Glucose into BY-2 Protoplasts. |
| Language: | English |
| Abstract: | In eukaryotic cells several pathways for the internalisation of plasma membrane proteins and extracellular cargo molecules exist. These endocytic pathways can be grouped into clathrin-dependent and clathrin-independent endocytosis. While the former one has been described to be involved in a variety of cellular processes in plants, the latter one has so far only been identified in animal and yeast cells. Here we show that internalisation of fluorescent glucose into BY-2 cells leads to accumulation of the sugar into compartments of the endocytic pathway. This endocytic uptake of glucose was not blocked by ikarugamycin (IKA), an inhibitor of clathrin-dependent endocytosis, suggesting a role for clathrin-independent endocytosis in glucose uptake. Investigations of fusion and fission of single vesicles by membrane capacitance measurements revealed a stimulation of endocytic activity by extracellular glucose. Glucose stimulated fission of vesicles was not affected by addition of IKA or block of clathrin coat formation by transient over-expression HUB1 (C-terminal part of the clathrin heavy chain). These data demonstrate that clathrin-independent endocytosis does exist in plant cells. This pathway may represent a common mechanism for the uptake of external nutrients. © 2011 The Authors. The Plant Journal© 2011 Blackwell Publishing Ltd. |
| Journal or Publication Title: | The Plant journal : for cell and molecular biology |
| Journal Volume: | 70 |
| Issue Number: | 4 |
| Divisions: | 10 Department of Biology > Plant Cell Biology ?? fb10_botanik ?? 10 Department of Biology |
| Date Deposited: | 10 Jan 2012 08:26 |
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