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The Arabidopsis aquaporin PIP1;2 rules cellular CO(2) uptake.

Uehlein, Norbert and Sperling, Henrik and Heckwolf, Marlies and Kaldenhoff, Ralf (2012):
The Arabidopsis aquaporin PIP1;2 rules cellular CO(2) uptake.
In: Plant, cell & environment, pp. 1077-1083, 35, (6), ISSN 1365-3040, [Article]

Abstract

The membrane CO(2) flux into Arabidopsis mesophyll cells was studied using a scanning pH microelectrode. Arabidopsis thaliana mesophyll cells were exposed to photosynthesis-triggering light intensities, which induced cellular CO(2) uptake. Data obtained on a AtPIP1;2 T-DNA insertion line indicated that under these conditions, cellular CO(2) transport was not limited by unstirred layer effects but was dependent on the expression of the aquaporin AtPIP1;2. Complementation of the AtPIP1;2 knockout restored membrane CO(2) transport levels to that of controls. The results provide new arguments for the ongoing debate about the validity of the lipid bilayer model system and the Meyer-Overton rule for cellular gas transport. In conclusion, we suggest a modified model of molecular gas transport mechanisms in living cells. © 2011 Blackwell Publishing Ltd.

Item Type: Article
Erschienen: 2012
Creators: Uehlein, Norbert and Sperling, Henrik and Heckwolf, Marlies and Kaldenhoff, Ralf
Title: The Arabidopsis aquaporin PIP1;2 rules cellular CO(2) uptake.
Language: English
Abstract:

The membrane CO(2) flux into Arabidopsis mesophyll cells was studied using a scanning pH microelectrode. Arabidopsis thaliana mesophyll cells were exposed to photosynthesis-triggering light intensities, which induced cellular CO(2) uptake. Data obtained on a AtPIP1;2 T-DNA insertion line indicated that under these conditions, cellular CO(2) transport was not limited by unstirred layer effects but was dependent on the expression of the aquaporin AtPIP1;2. Complementation of the AtPIP1;2 knockout restored membrane CO(2) transport levels to that of controls. The results provide new arguments for the ongoing debate about the validity of the lipid bilayer model system and the Meyer-Overton rule for cellular gas transport. In conclusion, we suggest a modified model of molecular gas transport mechanisms in living cells. © 2011 Blackwell Publishing Ltd.

Journal or Publication Title: Plant, cell & environment
Volume: 35
Number: 6
Divisions: 10 Department of Biology > Applied Plant Sciences
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10 Department of Biology
Date Deposited: 21 Dec 2011 13:44
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