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Lucifer Yellow stains a subpopulation of chicken retinoblasts preferentially 3 h after their last round of DNA synthesis.

Vollmer, G. and Layer, Paul G. (1986):
Lucifer Yellow stains a subpopulation of chicken retinoblasts preferentially 3 h after their last round of DNA synthesis.
In: Brain research, 392 (1-2), pp. 253-6, ISSN 0006-8993,
[Article]

Abstract

Observations of histological preparations of E4-E6 retinae suggested to us that Lucifer Yellow (LY) uptake occurs during a specific phase of the mitotic cell cycle. We examined this phenomenon more closely in intact E6 retinae. If we first exposed the tissues to a 20-min pulse of tritiated thymidine followed by LY-staining at different intervals, a distinct peak of double-labelled cells was found 3 h after exposure of the tissue to thymidine. In contrast, if staining with LY is followed by thymidine pulses, only few double-labelled cells are found. We conclude that a subpopulation of young neurons takes up LY specifically after their last round of DNA-synthesis.

Item Type: Article
Erschienen: 1986
Creators: Vollmer, G. and Layer, Paul G.
Title: Lucifer Yellow stains a subpopulation of chicken retinoblasts preferentially 3 h after their last round of DNA synthesis.
Language: English
Abstract:

Observations of histological preparations of E4-E6 retinae suggested to us that Lucifer Yellow (LY) uptake occurs during a specific phase of the mitotic cell cycle. We examined this phenomenon more closely in intact E6 retinae. If we first exposed the tissues to a 20-min pulse of tritiated thymidine followed by LY-staining at different intervals, a distinct peak of double-labelled cells was found 3 h after exposure of the tissue to thymidine. In contrast, if staining with LY is followed by thymidine pulses, only few double-labelled cells are found. We conclude that a subpopulation of young neurons takes up LY specifically after their last round of DNA-synthesis.

Journal or Publication Title: Brain research
Volume: 392
Number: 1-2
Divisions: 10 Department of Biology
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10 Department of Biology > Developmental Biology and Neurogenetics
Date Deposited: 21 Nov 2011 13:29
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