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Selective uptake of Lucifer Yellow into different cell populations of the developing chicken retina.

Layer, Paul G. ; Vollmer, G. ; Kotz, S. (1983)
Selective uptake of Lucifer Yellow into different cell populations of the developing chicken retina.
In: Neuroscience letters, 35 (3)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Whole chick embryo eyes at different stages of development were incubated in a solution of Lucifer Yellow (LY) and examined in frozen sections. Different cell populations selectively take up the dye during the course of development: from embryonic day 4 - day 8 of incubation (E4-E8) only a subpopulation of neuroepithelial cells are stained, which at E7-E8 are located mainly near the periphery between the prospective ora serrata and the outer edge of the retina. This staining disappears after E8. A second phase starts at E10, with a weak but specific staining of a small population of cells in the outer nuclear layer which becomes less pronounced by E12. Starting around E10.5 a bright staining of cells in the innermost part of the inner nuclear layer can be observed. Their size, shape and location suggest that most of them represent a subclass of amacrine cells (including all 'displaced amacrine cells'). Beginning at E15, the staining pattern becomes more complex. A single cell analysis of the percentage of LY-stained cells which are stained either before or after papain-treatment and dissociation of the intact tissue confirms and extends the histological findings.

Typ des Eintrags: Artikel
Erschienen: 1983
Autor(en): Layer, Paul G. ; Vollmer, G. ; Kotz, S.
Art des Eintrags: Bibliographie
Titel: Selective uptake of Lucifer Yellow into different cell populations of the developing chicken retina.
Sprache: Englisch
Publikationsjahr: 1983
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Neuroscience letters
Jahrgang/Volume einer Zeitschrift: 35
(Heft-)Nummer: 3
Kurzbeschreibung (Abstract):

Whole chick embryo eyes at different stages of development were incubated in a solution of Lucifer Yellow (LY) and examined in frozen sections. Different cell populations selectively take up the dye during the course of development: from embryonic day 4 - day 8 of incubation (E4-E8) only a subpopulation of neuroepithelial cells are stained, which at E7-E8 are located mainly near the periphery between the prospective ora serrata and the outer edge of the retina. This staining disappears after E8. A second phase starts at E10, with a weak but specific staining of a small population of cells in the outer nuclear layer which becomes less pronounced by E12. Starting around E10.5 a bright staining of cells in the innermost part of the inner nuclear layer can be observed. Their size, shape and location suggest that most of them represent a subclass of amacrine cells (including all 'displaced amacrine cells'). Beginning at E15, the staining pattern becomes more complex. A single cell analysis of the percentage of LY-stained cells which are stained either before or after papain-treatment and dissociation of the intact tissue confirms and extends the histological findings.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
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10 Fachbereich Biologie > Developmental Biology and Neurogenetics
Hinterlegungsdatum: 21 Nov 2011 13:20
Letzte Änderung: 05 Mär 2013 09:56
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