TU Darmstadt / ULB / TUbiblio

Butyrylcholinesterase regulates laminar retinogenesis of the chick embryo in vitro.

Willbold, E. ; Layer, Paul G. (1994)
Butyrylcholinesterase regulates laminar retinogenesis of the chick embryo in vitro.
In: European journal of cell biology, 64 (1)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

During chicken neurogenesis, the sequential expression of butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) between final cell proliferation and differentiation is functionally not understood. Recently, cholinesterases have been shown to regulate neurite growth in vitro. Here, we investigated the effects of inhibition of BChE on laminar histogenesis in retinospheroids that arise from dissociated embryonic chicken retinal cells in rotation culture. In the presence of the BChE inhibitor iso-OMPA (tetraisopropyl pyrophosphoramide), the number of spheroids/dish is increased, and their diameter is decreased by about 20%, corresponding to about 50% volume size. As a corollary, the course of histotypical differentiation is dramatically accelerated. Thus as a consequence of BChE inhibition both, organization of nuclear cell layers and of plexiform-like (neuropile) areas, as detected by an antibody to the fiber fasciculation protein F11, is temporally advanced by at least two days. Moreover, AChE is almost fully diminished in these areas. The results further demonstrate novel roles of cholinesterases during laminar histogenesis of coherent neural networks in vitro.

Typ des Eintrags: Artikel
Erschienen: 1994
Autor(en): Willbold, E. ; Layer, Paul G.
Art des Eintrags: Bibliographie
Titel: Butyrylcholinesterase regulates laminar retinogenesis of the chick embryo in vitro.
Sprache: Englisch
Publikationsjahr: 1994
Titel der Zeitschrift, Zeitung oder Schriftenreihe: European journal of cell biology
Jahrgang/Volume einer Zeitschrift: 64
(Heft-)Nummer: 1
Kurzbeschreibung (Abstract):

During chicken neurogenesis, the sequential expression of butyrylcholinesterase (BChE) and acetylcholinesterase (AChE) between final cell proliferation and differentiation is functionally not understood. Recently, cholinesterases have been shown to regulate neurite growth in vitro. Here, we investigated the effects of inhibition of BChE on laminar histogenesis in retinospheroids that arise from dissociated embryonic chicken retinal cells in rotation culture. In the presence of the BChE inhibitor iso-OMPA (tetraisopropyl pyrophosphoramide), the number of spheroids/dish is increased, and their diameter is decreased by about 20%, corresponding to about 50% volume size. As a corollary, the course of histotypical differentiation is dramatically accelerated. Thus as a consequence of BChE inhibition both, organization of nuclear cell layers and of plexiform-like (neuropile) areas, as detected by an antibody to the fiber fasciculation protein F11, is temporally advanced by at least two days. Moreover, AChE is almost fully diminished in these areas. The results further demonstrate novel roles of cholinesterases during laminar histogenesis of coherent neural networks in vitro.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
?? fb10_zoologie ??
10 Fachbereich Biologie > Developmental Biology and Neurogenetics
Hinterlegungsdatum: 21 Nov 2011 13:04
Letzte Änderung: 05 Mär 2013 09:56
PPN:
Export:
Suche nach Titel in: TUfind oder in Google
Frage zum Eintrag Frage zum Eintrag

Optionen (nur für Redakteure)
Redaktionelle Details anzeigen Redaktionelle Details anzeigen