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Expression of acetylcholinesterase (AChE) and aryl acylamidase (AAA) during early zebrafish embryogenesis.

Allebrandt, Karla V. ; Rajesh, Valmiki ; Layer, Paul G. :
Expression of acetylcholinesterase (AChE) and aryl acylamidase (AAA) during early zebrafish embryogenesis.
In: Chemico-biological interactions, 157-15 pp. 353-5. ISSN 0009-2797
[Artikel], (2005)

Kurzbeschreibung (Abstract)

In zebrafish, the onset of acetylcholinesterase (AChE) expression was detected by RT-PCR at 4 hpf (hours post-fertilization). The aryl acylamidase (AAA) associated with AChE, a serotonin sensitive activity with unknown physiological function, was significantly higher than the esterase activity on zebrafish embryos homogenates at 4-12 h development (test-t = 3.523; d.f. = 4). Remarkably, the ratio of AAA/AChE activity decreased 210-fold from 4 to 144 h development, indicating a distinct embryonic role of AAA during early embryogenesis. The AAA activity was sensitive to eserine and serotonin, ensuring its association with AChE. This is the first report of AAA activity on fish, establishing zebrafish as a model to study AAA on development.

Typ des Eintrags: Artikel
Erschienen: 2005
Autor(en): Allebrandt, Karla V. ; Rajesh, Valmiki ; Layer, Paul G.
Titel: Expression of acetylcholinesterase (AChE) and aryl acylamidase (AAA) during early zebrafish embryogenesis.
Sprache: Englisch
Kurzbeschreibung (Abstract):

In zebrafish, the onset of acetylcholinesterase (AChE) expression was detected by RT-PCR at 4 hpf (hours post-fertilization). The aryl acylamidase (AAA) associated with AChE, a serotonin sensitive activity with unknown physiological function, was significantly higher than the esterase activity on zebrafish embryos homogenates at 4-12 h development (test-t = 3.523; d.f. = 4). Remarkably, the ratio of AAA/AChE activity decreased 210-fold from 4 to 144 h development, indicating a distinct embryonic role of AAA during early embryogenesis. The AAA activity was sensitive to eserine and serotonin, ensuring its association with AChE. This is the first report of AAA activity on fish, establishing zebrafish as a model to study AAA on development.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Chemico-biological interactions
Band: 157-15
Fachbereich(e)/-gebiet(e): Fachbereich Biologie, Biology
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Fachbereich Biologie, Biology > Entwicklungsbiologie und Neurogenetik, Developmental Biology and Neurogenetics
Hinterlegungsdatum: 21 Nov 2011 10:31
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