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Mutations in 16S rRNA that affect UGA (stop codon)-directed translation termination.

Göringer, H. Ulrich ; Hijazi, K. A. ; Murgola, E. J. ; Dahlberg, A. E. :
Mutations in 16S rRNA that affect UGA (stop codon)-directed translation termination.
In: Proceedings of the National Academy of Sciences of the United States of America, 88 (15) pp. 6603-7. ISSN 0027-8424
[Artikel], (1991)

Kurzbeschreibung (Abstract)

Site-directed mutagenesis was performed on a sequence motif within the 3' major domain of Escherichia coli 16S rRNA shown previously to be important for peptide chain termination. Analysis of stop codon suppression by the various mutants showed an exclusive response to UGA stop signals, which was correlated directly with the continuity of one or the other of two tandem complementary UCA sequences (bases 1199-1204). Since no other structural features of the mutated ribosomes were hampered and the translation initiation and elongation events functioned properly, we propose that a direct interaction occurs between the UGA stop codon on the mRNA and the 16S rRNA UCA motif as one of the initial events of UGA-dependent peptide chain termination. These results provide evidence that base pairing between rRNA and mRNA plays a direct role in termination, as it has already been shown to do for initiation and elongation.

Typ des Eintrags: Artikel
Erschienen: 1991
Autor(en): Göringer, H. Ulrich ; Hijazi, K. A. ; Murgola, E. J. ; Dahlberg, A. E.
Titel: Mutations in 16S rRNA that affect UGA (stop codon)-directed translation termination.
Sprache: Englisch
Kurzbeschreibung (Abstract):

Site-directed mutagenesis was performed on a sequence motif within the 3' major domain of Escherichia coli 16S rRNA shown previously to be important for peptide chain termination. Analysis of stop codon suppression by the various mutants showed an exclusive response to UGA stop signals, which was correlated directly with the continuity of one or the other of two tandem complementary UCA sequences (bases 1199-1204). Since no other structural features of the mutated ribosomes were hampered and the translation initiation and elongation events functioned properly, we propose that a direct interaction occurs between the UGA stop codon on the mRNA and the 16S rRNA UCA motif as one of the initial events of UGA-dependent peptide chain termination. These results provide evidence that base pairing between rRNA and mRNA plays a direct role in termination, as it has already been shown to do for initiation and elongation.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Proceedings of the National Academy of Sciences of the United States of America
Band: 88
(Heft-)Nummer: 15
Fachbereich(e)/-gebiet(e): Fachbereich Biologie, Biology > Genregulation und RNA-Therapeutika, Postranscriptional Gene Regulation and RNA Therapeutics
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Fachbereich Biologie, Biology
Hinterlegungsdatum: 03 Nov 2011 13:53
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