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The formation of mitochondrial ribonucleoprotein complexes involving guide RNA molecules in Trypanosoma brucei.

Göringer, H. Ulrich ; Koslowsky, D. J. ; Morales, T. H. ; Stuart, K. :
The formation of mitochondrial ribonucleoprotein complexes involving guide RNA molecules in Trypanosoma brucei.
In: Proceedings of the National Academy of Sciences of the United States of America, 91 (5) pp. 1776-80. ISSN 0027-8424
[Artikel], (1994)

Kurzbeschreibung (Abstract)

Transcripts from mitochondrial (maxicircle) genes of kinetoplastid organisms undergo RNA editing characterized by a series of reactions that insert and delete uridine nucleotides within the sequence of the pre-mRNAs. Guide RNAs, which complement fully edited mRNAs, provide the information for the edited sequence by an unknown mechanism. We report here that guide RNA molecules associate with other mitochondrial components to form four specific, stable ribonucleoprotein complexes. The complexes form very rapidly at a low monovalent cation concentration, and their formation is blocked by heparin or pretreatment of the mitochondrial lysate with SDS. ATP hydrolysis is not required but slightly stimulates complex association up to concentrations of 5 mM. The results are suggestive of a sequential assembly of the ribonucleoprotein complexes, and their possible involvement during the kinetoplastid RNA editing is discussed.

Typ des Eintrags: Artikel
Erschienen: 1994
Autor(en): Göringer, H. Ulrich ; Koslowsky, D. J. ; Morales, T. H. ; Stuart, K.
Titel: The formation of mitochondrial ribonucleoprotein complexes involving guide RNA molecules in Trypanosoma brucei.
Sprache: Englisch
Kurzbeschreibung (Abstract):

Transcripts from mitochondrial (maxicircle) genes of kinetoplastid organisms undergo RNA editing characterized by a series of reactions that insert and delete uridine nucleotides within the sequence of the pre-mRNAs. Guide RNAs, which complement fully edited mRNAs, provide the information for the edited sequence by an unknown mechanism. We report here that guide RNA molecules associate with other mitochondrial components to form four specific, stable ribonucleoprotein complexes. The complexes form very rapidly at a low monovalent cation concentration, and their formation is blocked by heparin or pretreatment of the mitochondrial lysate with SDS. ATP hydrolysis is not required but slightly stimulates complex association up to concentrations of 5 mM. The results are suggestive of a sequential assembly of the ribonucleoprotein complexes, and their possible involvement during the kinetoplastid RNA editing is discussed.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Proceedings of the National Academy of Sciences of the United States of America
Band: 91
(Heft-)Nummer: 5
Fachbereich(e)/-gebiet(e): Fachbereich Biologie, Biology > Genregulation und RNA-Therapeutika, Postranscriptional Gene Regulation and RNA Therapeutics
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Fachbereich Biologie, Biology
Hinterlegungsdatum: 03 Nov 2011 13:49
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