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Association of guide RNA binding protein gBP21 with active RNA editing complexes in Trypanosoma brucei.

Allen, T. E. ; Heidmann, S. ; Reed, R. ; Myler, P. J. ; Göringer, H. Ulrich ; Stuart, K. D. :
Association of guide RNA binding protein gBP21 with active RNA editing complexes in Trypanosoma brucei.
In: Molecular and cellular biology, 18 (10) pp. 6014-22. ISSN 0270-7306
[Artikel], (1998)

Kurzbeschreibung (Abstract)

RNA editing in Trypanosoma brucei mitochondria produces mature mRNAs by a series of enzyme-catalyzed reactions that specifically insert or delete uridylates in association with a macromolecular complex. Using a mitochondrial fraction enriched for in vitro RNA editing activity, we produced several monoclonal antibodies that are specific for a 21-kDa guide RNA (gRNA) binding protein initially identified by UV cross-linking. Immunofluorescence studies localize the protein to the mitochondrion, with a preference for the kinetoplast. The antibodies cause a supershift of previously identified gRNA-specific ribonucleoprotein complexes and immunoprecipitate in vitro RNA editing activities that insert and delete uridylates. The immunoprecipitated material also contains gRNA-specific endoribonuclease, terminal uridylyltransferase, and RNA ligase activities as well as gRNA and both edited and unedited mRNA. The immunoprecipitate contains numerous proteins, of which the 21-kDa protein, a 90-kDa protein, and novel 55- and 16-kDa proteins can be UV cross-linked to gRNA. These studies indicate that the 21-kDa protein associates with the ribonucleoprotein complex (or complexes) that catalyze RNA editing.

Typ des Eintrags: Artikel
Erschienen: 1998
Autor(en): Allen, T. E. ; Heidmann, S. ; Reed, R. ; Myler, P. J. ; Göringer, H. Ulrich ; Stuart, K. D.
Titel: Association of guide RNA binding protein gBP21 with active RNA editing complexes in Trypanosoma brucei.
Sprache: Englisch
Kurzbeschreibung (Abstract):

RNA editing in Trypanosoma brucei mitochondria produces mature mRNAs by a series of enzyme-catalyzed reactions that specifically insert or delete uridylates in association with a macromolecular complex. Using a mitochondrial fraction enriched for in vitro RNA editing activity, we produced several monoclonal antibodies that are specific for a 21-kDa guide RNA (gRNA) binding protein initially identified by UV cross-linking. Immunofluorescence studies localize the protein to the mitochondrion, with a preference for the kinetoplast. The antibodies cause a supershift of previously identified gRNA-specific ribonucleoprotein complexes and immunoprecipitate in vitro RNA editing activities that insert and delete uridylates. The immunoprecipitated material also contains gRNA-specific endoribonuclease, terminal uridylyltransferase, and RNA ligase activities as well as gRNA and both edited and unedited mRNA. The immunoprecipitate contains numerous proteins, of which the 21-kDa protein, a 90-kDa protein, and novel 55- and 16-kDa proteins can be UV cross-linked to gRNA. These studies indicate that the 21-kDa protein associates with the ribonucleoprotein complex (or complexes) that catalyze RNA editing.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Molecular and cellular biology
Band: 18
(Heft-)Nummer: 10
Fachbereich(e)/-gebiet(e): Fachbereich Biologie, Biology > Genregulation und RNA-Therapeutika, Postranscriptional Gene Regulation and RNA Therapeutics
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Fachbereich Biologie, Biology
Hinterlegungsdatum: 03 Nov 2011 13:20
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