Allen, T. E. ; Heidmann, S. ; Reed, R. ; Myler, P. J. ; Göringer, H. Ulrich ; Stuart, K. D. (1998)
Association of guide RNA binding protein gBP21 with active RNA editing complexes in Trypanosoma brucei.
In: Molecular and cellular biology, 18 (10)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
RNA editing in Trypanosoma brucei mitochondria produces mature mRNAs by a series of enzyme-catalyzed reactions that specifically insert or delete uridylates in association with a macromolecular complex. Using a mitochondrial fraction enriched for in vitro RNA editing activity, we produced several monoclonal antibodies that are specific for a 21-kDa guide RNA (gRNA) binding protein initially identified by UV cross-linking. Immunofluorescence studies localize the protein to the mitochondrion, with a preference for the kinetoplast. The antibodies cause a supershift of previously identified gRNA-specific ribonucleoprotein complexes and immunoprecipitate in vitro RNA editing activities that insert and delete uridylates. The immunoprecipitated material also contains gRNA-specific endoribonuclease, terminal uridylyltransferase, and RNA ligase activities as well as gRNA and both edited and unedited mRNA. The immunoprecipitate contains numerous proteins, of which the 21-kDa protein, a 90-kDa protein, and novel 55- and 16-kDa proteins can be UV cross-linked to gRNA. These studies indicate that the 21-kDa protein associates with the ribonucleoprotein complex (or complexes) that catalyze RNA editing.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1998 |
| Autor(en): | Allen, T. E. ; Heidmann, S. ; Reed, R. ; Myler, P. J. ; Göringer, H. Ulrich ; Stuart, K. D. |
| Art des Eintrags: | Bibliographie |
| Titel: | Association of guide RNA binding protein gBP21 with active RNA editing complexes in Trypanosoma brucei. |
| Sprache: | Englisch |
| Publikationsjahr: | 1998 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Molecular and cellular biology |
| Jahrgang/Volume einer Zeitschrift: | 18 |
| (Heft-)Nummer: | 10 |
| Kurzbeschreibung (Abstract): | RNA editing in Trypanosoma brucei mitochondria produces mature mRNAs by a series of enzyme-catalyzed reactions that specifically insert or delete uridylates in association with a macromolecular complex. Using a mitochondrial fraction enriched for in vitro RNA editing activity, we produced several monoclonal antibodies that are specific for a 21-kDa guide RNA (gRNA) binding protein initially identified by UV cross-linking. Immunofluorescence studies localize the protein to the mitochondrion, with a preference for the kinetoplast. The antibodies cause a supershift of previously identified gRNA-specific ribonucleoprotein complexes and immunoprecipitate in vitro RNA editing activities that insert and delete uridylates. The immunoprecipitated material also contains gRNA-specific endoribonuclease, terminal uridylyltransferase, and RNA ligase activities as well as gRNA and both edited and unedited mRNA. The immunoprecipitate contains numerous proteins, of which the 21-kDa protein, a 90-kDa protein, and novel 55- and 16-kDa proteins can be UV cross-linked to gRNA. These studies indicate that the 21-kDa protein associates with the ribonucleoprotein complex (or complexes) that catalyze RNA editing. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Genregulation und RNA-Therapeutika ?? fb10_mikrobiologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 03 Nov 2011 13:20 |
| Letzte Änderung: | 05 Mär 2013 09:55 |
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