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Promoter analysis of the bli-7/eas gene.

Kaldenhoff, Ralf ; Russo, V. E. (1993)
Promoter analysis of the bli-7/eas gene.
In: Current genetics, 24 (5)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Expression of the Neurospora crassa gene bli-7, (identical with eas, and ccg-2), is induced by blue light, as well as glucose- or nitrogen-starvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of foreign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducibility of the gene, in response to the three inducing conditions mentioned, is lost by eliminating the region between -1498 bp and -1017 bp upstream of the transcription start point. A segment with an apparently negative effect was found between -595 bp and -429 bp, as well as a stretch of DNA from -429 bp to -380 bp which may exert a positive influence after light induction.

Typ des Eintrags: Artikel
Erschienen: 1993
Autor(en): Kaldenhoff, Ralf ; Russo, V. E.
Art des Eintrags: Bibliographie
Titel: Promoter analysis of the bli-7/eas gene.
Sprache: Englisch
Publikationsjahr: 1993
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Current genetics
Jahrgang/Volume einer Zeitschrift: 24
(Heft-)Nummer: 5
Kurzbeschreibung (Abstract):

Expression of the Neurospora crassa gene bli-7, (identical with eas, and ccg-2), is induced by blue light, as well as glucose- or nitrogen-starvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of foreign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducibility of the gene, in response to the three inducing conditions mentioned, is lost by eliminating the region between -1498 bp and -1017 bp upstream of the transcription start point. A segment with an apparently negative effect was found between -595 bp and -429 bp, as well as a stretch of DNA from -429 bp to -380 bp which may exert a positive influence after light induction.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie > Applied Plant Sciences
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10 Fachbereich Biologie
Hinterlegungsdatum: 31 Aug 2011 13:18
Letzte Änderung: 05 Mär 2013 09:54
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