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Promoter analysis of the bli-7/eas gene.

Kaldenhoff, Ralf and Russo, V. E. (1993):
Promoter analysis of the bli-7/eas gene.
In: Current genetics, pp. 394-9, 24, (5), ISSN 0172-8083,
[Article]

Abstract

Expression of the Neurospora crassa gene bli-7, (identical with eas, and ccg-2), is induced by blue light, as well as glucose- or nitrogen-starvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of foreign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducibility of the gene, in response to the three inducing conditions mentioned, is lost by eliminating the region between -1498 bp and -1017 bp upstream of the transcription start point. A segment with an apparently negative effect was found between -595 bp and -429 bp, as well as a stretch of DNA from -429 bp to -380 bp which may exert a positive influence after light induction.

Item Type: Article
Erschienen: 1993
Creators: Kaldenhoff, Ralf and Russo, V. E.
Title: Promoter analysis of the bli-7/eas gene.
Language: English
Abstract:

Expression of the Neurospora crassa gene bli-7, (identical with eas, and ccg-2), is induced by blue light, as well as glucose- or nitrogen-starvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of foreign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducibility of the gene, in response to the three inducing conditions mentioned, is lost by eliminating the region between -1498 bp and -1017 bp upstream of the transcription start point. A segment with an apparently negative effect was found between -595 bp and -429 bp, as well as a stretch of DNA from -429 bp to -380 bp which may exert a positive influence after light induction.

Journal or Publication Title: Current genetics
Volume: 24
Number: 5
Divisions: 10 Department of Biology > Applied Plant Sciences
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10 Department of Biology
Date Deposited: 31 Aug 2011 13:18
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