Biela, A. ; Grote, K. ; Otto, Beate ; Hoth, S. ; Hedrich, R. ; Kaldenhoff, Ralf (1999)
The Nicotiana tabacum plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol.
In: The Plant journal : for cell and molecular biology, 18 (5)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
A new aquaporin from Nicotiana tabacum (cv. Samsun) was characterized. It shares sequence homology to the Arabidopsis thaliana PIP1 protein family. By two-phase partitioning and immunoblot analysis, plasma membrane localization could be demonstrated. The corresponding mRNA is highly abundant in roots and flowers, while it is rarely expressed in leaves and stems. Functional expression in Xenopus oocytes revealed that NtAQP1 can mediate glycerol transport in addition to water flow. However, NtAQP1 is impermeable for Na+, K+ and Cl- ions. The water permeability and selectivity could not be modulated by addition of mercurials or the activity of cAMP-dependent protein kinases.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1999 |
| Autor(en): | Biela, A. ; Grote, K. ; Otto, Beate ; Hoth, S. ; Hedrich, R. ; Kaldenhoff, Ralf |
| Art des Eintrags: | Bibliographie |
| Titel: | The Nicotiana tabacum plasma membrane aquaporin NtAQP1 is mercury-insensitive and permeable for glycerol. |
| Sprache: | Englisch |
| Publikationsjahr: | 1999 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | The Plant journal : for cell and molecular biology |
| Jahrgang/Volume einer Zeitschrift: | 18 |
| (Heft-)Nummer: | 5 |
| Kurzbeschreibung (Abstract): | A new aquaporin from Nicotiana tabacum (cv. Samsun) was characterized. It shares sequence homology to the Arabidopsis thaliana PIP1 protein family. By two-phase partitioning and immunoblot analysis, plasma membrane localization could be demonstrated. The corresponding mRNA is highly abundant in roots and flowers, while it is rarely expressed in leaves and stems. Functional expression in Xenopus oocytes revealed that NtAQP1 can mediate glycerol transport in addition to water flow. However, NtAQP1 is impermeable for Na+, K+ and Cl- ions. The water permeability and selectivity could not be modulated by addition of mercurials or the activity of cAMP-dependent protein kinases. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Applied Plant Sciences ?? fb10_botanik ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 31 Aug 2011 13:10 |
| Letzte Änderung: | 05 Mär 2013 09:54 |
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