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Cell-specific expression of the mercury-insensitive plasma-membrane aquaporin NtAQP1 from Nicotiana tabacum.

Otto, Beate ; Kaldenhoff, Ralf (2000)
Cell-specific expression of the mercury-insensitive plasma-membrane aquaporin NtAQP1 from Nicotiana tabacum.
In: Planta, 211 (2)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

The aquaporin NtAQP1 from Nicotiana tabacum L. is insensitive to heavy-metal ions. In addition to water, the transport of urea or glycerol is facilitated by this plasma-membrane-located water channel. Northern hybridization and whole-mount in situ hybridization revealed a high steady-state level of NtAQP1-RNA in roots, a decreased content in shoots and a low content in leaves. By immunolocalization with an antibody targeted to the N-terminus of the aquaporin, the localization of NtAQP1-protein at sites of expected high water transport rates from and to the apoplast or symplast could be demonstrated. The specific pattern of NtAQP1 distribution in petioles strongly indicates a transcellular movement of water.

Typ des Eintrags: Artikel
Erschienen: 2000
Autor(en): Otto, Beate ; Kaldenhoff, Ralf
Art des Eintrags: Bibliographie
Titel: Cell-specific expression of the mercury-insensitive plasma-membrane aquaporin NtAQP1 from Nicotiana tabacum.
Sprache: Englisch
Publikationsjahr: 2000
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Planta
Jahrgang/Volume einer Zeitschrift: 211
(Heft-)Nummer: 2
Kurzbeschreibung (Abstract):

The aquaporin NtAQP1 from Nicotiana tabacum L. is insensitive to heavy-metal ions. In addition to water, the transport of urea or glycerol is facilitated by this plasma-membrane-located water channel. Northern hybridization and whole-mount in situ hybridization revealed a high steady-state level of NtAQP1-RNA in roots, a decreased content in shoots and a low content in leaves. By immunolocalization with an antibody targeted to the N-terminus of the aquaporin, the localization of NtAQP1-protein at sites of expected high water transport rates from and to the apoplast or symplast could be demonstrated. The specific pattern of NtAQP1 distribution in petioles strongly indicates a transcellular movement of water.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie > Applied Plant Sciences
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10 Fachbereich Biologie
Hinterlegungsdatum: 31 Aug 2011 13:08
Letzte Änderung: 05 Mär 2013 09:54
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