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PIP1 plasma membrane aquaporins in tobacco: from cellular effects to function in plants.

Siefritz, Franka and Tyree, Melvin T. and Lovisolo, Claudio and Schubert, Andrea and Kaldenhoff, Ralf (2002):
PIP1 plasma membrane aquaporins in tobacco: from cellular effects to function in plants.
In: The Plant cell, pp. 869-76, 14, (4), ISSN 1040-4651, [Article]

Abstract

The molecular functions of several aquaporins are well characterized (e.g., by analysis of aquaporin-expressing Xenopus oocytes). However, their significance in the physiology of water transport in multicellular organisms remains uncertain. The tobacco plasma membrane aquaporin NtAQP1 was used to elucidate this issue. By comparing antisense plants that were inhibited in NtAQP1 expression with control plants, we found evidence for NtAQP1 function in cellular and whole-plant water relations. The consequences of a decrease in cellular water permeability were determined by measurement of transpiration rate and stem and leaf water potential as well as growth experiments under extreme soil water depletion. Plants impaired in NtAQP1 expression showed reduced root hydraulic conductivity and lower water stress resistance. In conclusion, our results emphasize the importance of symplastic aquaporin-mediated water transport in whole-plant water relations.

Item Type: Article
Erschienen: 2002
Creators: Siefritz, Franka and Tyree, Melvin T. and Lovisolo, Claudio and Schubert, Andrea and Kaldenhoff, Ralf
Title: PIP1 plasma membrane aquaporins in tobacco: from cellular effects to function in plants.
Language: English
Abstract:

The molecular functions of several aquaporins are well characterized (e.g., by analysis of aquaporin-expressing Xenopus oocytes). However, their significance in the physiology of water transport in multicellular organisms remains uncertain. The tobacco plasma membrane aquaporin NtAQP1 was used to elucidate this issue. By comparing antisense plants that were inhibited in NtAQP1 expression with control plants, we found evidence for NtAQP1 function in cellular and whole-plant water relations. The consequences of a decrease in cellular water permeability were determined by measurement of transpiration rate and stem and leaf water potential as well as growth experiments under extreme soil water depletion. Plants impaired in NtAQP1 expression showed reduced root hydraulic conductivity and lower water stress resistance. In conclusion, our results emphasize the importance of symplastic aquaporin-mediated water transport in whole-plant water relations.

Journal or Publication Title: The Plant cell
Volume: 14
Number: 4
Divisions: 10 Department of Biology > Applied Plant Sciences
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10 Department of Biology
Date Deposited: 31 Aug 2011 12:44
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