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Modes of exocytotic and endocytotic events in tobacco BY-2 protoplasts.

Bandmann, Vera ; Kreft, Marko ; Homann, Ulrike (2011)
Modes of exocytotic and endocytotic events in tobacco BY-2 protoplasts.
In: Molecular plant, 4 (2)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

To analyze the kinetics and size of single exo- and endocytotic events in BY-2 protoplasts, we employed cell-attached membrane capacitance measurements. These measurements revealed different modes of fusion and fission of single vesicles. In about half of the observed exocytotic events, fusion occurred transiently, which facilitates rapid recycling of vesicles. In addition, transient sequential or multi-vesicular exocytosis observed in some recordings can contribute to an increase in efficiency of secretory product release. Microscopic analysis of the timescale of cellulose and pectin deposition in protoplasts demonstrates that rebuilding of the cell wall starts soon after isolation of protoplasts and that transient fusion events can fully account for secretion of the required soluble material. The capacitance measurements also allowed us to investigate formation of the fusion pore. We speculate that regulation of secretion may involve control of the length and/or size of fusion pore opening. Together, the different kinetic modes of exo- and endocytosis revealed by capacitance measurements underline the complexity of this process in plants and provide a basis for future research into the underlying mechanisms. The fact that similar fusion/fission kinetics are present in plant and animal cells suggests that many of these mechanisms are highly conserved among eukaryotes.

Typ des Eintrags: Artikel
Erschienen: 2011
Autor(en): Bandmann, Vera ; Kreft, Marko ; Homann, Ulrike
Art des Eintrags: Bibliographie
Titel: Modes of exocytotic and endocytotic events in tobacco BY-2 protoplasts.
Sprache: Englisch
Publikationsjahr: 2011
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Molecular plant
Jahrgang/Volume einer Zeitschrift: 4
(Heft-)Nummer: 2
Kurzbeschreibung (Abstract):

To analyze the kinetics and size of single exo- and endocytotic events in BY-2 protoplasts, we employed cell-attached membrane capacitance measurements. These measurements revealed different modes of fusion and fission of single vesicles. In about half of the observed exocytotic events, fusion occurred transiently, which facilitates rapid recycling of vesicles. In addition, transient sequential or multi-vesicular exocytosis observed in some recordings can contribute to an increase in efficiency of secretory product release. Microscopic analysis of the timescale of cellulose and pectin deposition in protoplasts demonstrates that rebuilding of the cell wall starts soon after isolation of protoplasts and that transient fusion events can fully account for secretion of the required soluble material. The capacitance measurements also allowed us to investigate formation of the fusion pore. We speculate that regulation of secretion may involve control of the length and/or size of fusion pore opening. Together, the different kinetic modes of exo- and endocytosis revealed by capacitance measurements underline the complexity of this process in plants and provide a basis for future research into the underlying mechanisms. The fact that similar fusion/fission kinetics are present in plant and animal cells suggests that many of these mechanisms are highly conserved among eukaryotes.

Fachbereich(e)/-gebiet(e): ?? fb10_botanik ??
10 Fachbereich Biologie > Pflanzliche Zellbiologie
10 Fachbereich Biologie
Hinterlegungsdatum: 31 Aug 2011 08:36
Letzte Änderung: 05 Mär 2013 09:54
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