TU Darmstadt / ULB / TUbiblio

Modes of exocytotic and endocytotic events in tobacco BY-2 protoplasts.

Bandmann, Vera and Kreft, Marko and Homann, Ulrike :
Modes of exocytotic and endocytotic events in tobacco BY-2 protoplasts.
In: Molecular plant, 4 (2) pp. 241-51. ISSN 1752-9867
[Article] , (2011)

Abstract

To analyze the kinetics and size of single exo- and endocytotic events in BY-2 protoplasts, we employed cell-attached membrane capacitance measurements. These measurements revealed different modes of fusion and fission of single vesicles. In about half of the observed exocytotic events, fusion occurred transiently, which facilitates rapid recycling of vesicles. In addition, transient sequential or multi-vesicular exocytosis observed in some recordings can contribute to an increase in efficiency of secretory product release. Microscopic analysis of the timescale of cellulose and pectin deposition in protoplasts demonstrates that rebuilding of the cell wall starts soon after isolation of protoplasts and that transient fusion events can fully account for secretion of the required soluble material. The capacitance measurements also allowed us to investigate formation of the fusion pore. We speculate that regulation of secretion may involve control of the length and/or size of fusion pore opening. Together, the different kinetic modes of exo- and endocytosis revealed by capacitance measurements underline the complexity of this process in plants and provide a basis for future research into the underlying mechanisms. The fact that similar fusion/fission kinetics are present in plant and animal cells suggests that many of these mechanisms are highly conserved among eukaryotes.

Item Type: Article
Erschienen: 2011
Creators: Bandmann, Vera and Kreft, Marko and Homann, Ulrike
Title: Modes of exocytotic and endocytotic events in tobacco BY-2 protoplasts.
Language: English
Abstract:

To analyze the kinetics and size of single exo- and endocytotic events in BY-2 protoplasts, we employed cell-attached membrane capacitance measurements. These measurements revealed different modes of fusion and fission of single vesicles. In about half of the observed exocytotic events, fusion occurred transiently, which facilitates rapid recycling of vesicles. In addition, transient sequential or multi-vesicular exocytosis observed in some recordings can contribute to an increase in efficiency of secretory product release. Microscopic analysis of the timescale of cellulose and pectin deposition in protoplasts demonstrates that rebuilding of the cell wall starts soon after isolation of protoplasts and that transient fusion events can fully account for secretion of the required soluble material. The capacitance measurements also allowed us to investigate formation of the fusion pore. We speculate that regulation of secretion may involve control of the length and/or size of fusion pore opening. Together, the different kinetic modes of exo- and endocytosis revealed by capacitance measurements underline the complexity of this process in plants and provide a basis for future research into the underlying mechanisms. The fact that similar fusion/fission kinetics are present in plant and animal cells suggests that many of these mechanisms are highly conserved among eukaryotes.

Journal or Publication Title: Molecular plant
Volume: 4
Number: 2
Divisions: ?? fb10_botanik ??
10 Department of Biology > Plant Cell Biology
10 Department of Biology
Date Deposited: 31 Aug 2011 08:36
Export:

Optionen (nur für Redakteure)

View Item View Item