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Histone acetylation controls the inactive X chromosome replication dynamics.

Casas-Delucchi, Corella S. and Brero, Alessandro and Rahn, Hans-Peter and Solovei, Irina and Wutz, Anton and Cremer, Thomas and Leonhardt, Heinrich and Cardoso, M. Cristina :
Histone acetylation controls the inactive X chromosome replication dynamics.
[Online-Edition: http://www.cardoso-lab.org/publications/CasasDelucchi_2011.p...]
In: Nature communications, 2 p. 222. ISSN 2041-1723
[Article] , (2011)

Official URL: http://www.cardoso-lab.org/publications/CasasDelucchi_2011.p...

Abstract

In mammals, dosage compensation between male and female cells is achieved by inactivating one female X chromosome (Xi). Late replication of Xi was proposed to be involved in the maintenance of its silenced state. Here, we show a highly synchronous replication of the Xi within 1 to 2 h during early-mid S-phase by following DNA replication in living mammalian cells with green fluorescent protein-tagged replication proteins. The Xi was replicated before or concomitant with perinuclear or perinucleolar facultative heterochromatin and before constitutive heterochromatin. Ectopic expression of the X-inactive-specific transcript (Xist) gene from an autosome imposed the same synchronous replication pattern. We used mutations and chemical inhibition affecting different epigenetic marks as well as inducible Xist expression and we demonstrate that histone hypoacetylation has a key role in controlling Xi replication. The epigenetically controlled, highly coordinated replication of the Xi is reminiscent of embryonic genome replication in flies and frogs before genome activation and might be a common feature of transcriptionally silent chromatin.

Item Type: Article
Erschienen: 2011
Creators: Casas-Delucchi, Corella S. and Brero, Alessandro and Rahn, Hans-Peter and Solovei, Irina and Wutz, Anton and Cremer, Thomas and Leonhardt, Heinrich and Cardoso, M. Cristina
Title: Histone acetylation controls the inactive X chromosome replication dynamics.
Language: English
Abstract:

In mammals, dosage compensation between male and female cells is achieved by inactivating one female X chromosome (Xi). Late replication of Xi was proposed to be involved in the maintenance of its silenced state. Here, we show a highly synchronous replication of the Xi within 1 to 2 h during early-mid S-phase by following DNA replication in living mammalian cells with green fluorescent protein-tagged replication proteins. The Xi was replicated before or concomitant with perinuclear or perinucleolar facultative heterochromatin and before constitutive heterochromatin. Ectopic expression of the X-inactive-specific transcript (Xist) gene from an autosome imposed the same synchronous replication pattern. We used mutations and chemical inhibition affecting different epigenetic marks as well as inducible Xist expression and we demonstrate that histone hypoacetylation has a key role in controlling Xi replication. The epigenetically controlled, highly coordinated replication of the Xi is reminiscent of embryonic genome replication in flies and frogs before genome activation and might be a common feature of transcriptionally silent chromatin.

Journal or Publication Title: Nature communications
Volume: 2
Divisions: 10 Department of Biology > Cell Biology and Epigenetics
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10 Department of Biology
Date Deposited: 27 Jul 2011 10:10
Official URL: http://www.cardoso-lab.org/publications/CasasDelucchi_2011.p...
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