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Ca2+-sensitive and Ca2+-insensitive exocytosis in maize coleoptile protoplasts.

Thiel, Gerhard and Sutter, J. U. and Homann, Ulrike (2000):
Ca2+-sensitive and Ca2+-insensitive exocytosis in maize coleoptile protoplasts.
In: Pflügers Archiv : European journal of physiology, pp. R152-3, 439, (3 Supp), ISSN 0031-6768, [Article]

Abstract

Ca2+ and osmotic driven extension of the surface area of maize coleoptile protoplasts was investigated using capacitance measurements and photolysis of the caged compound DM-nitrophen. Protoplasts responded to an elevation of cytoplasmic Ca2+ (Ca(i)) with a rapid burst in capacitance reaching a maximal increase of 1.3+/-1.1% over the resting cell capacitance. Subsequent lowering of the osmotic potential in the external medium by 210 mosmol caused a further increase in Cm by 26+/-6%. These data indicate two independent pathways for insertion of membrane into the plasma membrane. One is driven by Ca(i) and recruits membrane from a small pool. The osmotic evoked rise in surface area draws membrane from a much larger reservoir and may be driven by membrane tension.

Item Type: Article
Erschienen: 2000
Creators: Thiel, Gerhard and Sutter, J. U. and Homann, Ulrike
Title: Ca2+-sensitive and Ca2+-insensitive exocytosis in maize coleoptile protoplasts.
Language: English
Abstract:

Ca2+ and osmotic driven extension of the surface area of maize coleoptile protoplasts was investigated using capacitance measurements and photolysis of the caged compound DM-nitrophen. Protoplasts responded to an elevation of cytoplasmic Ca2+ (Ca(i)) with a rapid burst in capacitance reaching a maximal increase of 1.3+/-1.1% over the resting cell capacitance. Subsequent lowering of the osmotic potential in the external medium by 210 mosmol caused a further increase in Cm by 26+/-6%. These data indicate two independent pathways for insertion of membrane into the plasma membrane. One is driven by Ca(i) and recruits membrane from a small pool. The osmotic evoked rise in surface area draws membrane from a much larger reservoir and may be driven by membrane tension.

Journal or Publication Title: Pflügers Archiv : European journal of physiology
Volume: 439
Number: 3 Supp
Divisions: 10 Department of Biology
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10 Department of Biology > Plant Membrane Biophysics
10 Department of Biology > Plant Cell Biology
Date Deposited: 22 Jun 2011 07:39
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