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A potassium channel protein encoded by chlorella virus PBCV-1.

Plugge, B. ; Gazzarrini, S. ; Nelson, M. ; Cerana, R. ; Etten, James L. van ; Derst, C. ; DiFrancesco, D. ; Moroni, Anna ; Thiel, Gerhard (2000):
A potassium channel protein encoded by chlorella virus PBCV-1.
In: Science (New York, N.Y.), 287 (5458), pp. 1641-4. ISSN 0036-8075,
[Article]

Abstract

The large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus life cycle.

Item Type: Article
Erschienen: 2000
Creators: Plugge, B. ; Gazzarrini, S. ; Nelson, M. ; Cerana, R. ; Etten, James L. van ; Derst, C. ; DiFrancesco, D. ; Moroni, Anna ; Thiel, Gerhard
Title: A potassium channel protein encoded by chlorella virus PBCV-1.
Language: English
Abstract:

The large chlorella virus PBCV-1, which contains double-stranded DNA (dsDNA), encodes a 94-codon open reading frame (ORF) that contains a motif resembling the signature sequence of the pore domain of potassium channel proteins. Phylogenetic analyses of the encoded protein, Kcv, indicate a previously unidentified type of potassium channel. The messenger RNA encoded by the ORF leads to functional expression of a potassium-selective conductance in Xenopus laevis oocytes. The channel blockers amantadine and barium, but not cesium, inhibit this conductance, in addition to virus plaque formation. Thus, PBCV-1 encodes the first known viral protein that functions as a potassium-selective channel and is essential in the virus life cycle.

Journal or Publication Title: Science (New York, N.Y.)
Journal volume: 287
Number: 5458
Divisions: 10 Department of Biology
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10 Department of Biology > Plant Membrane Biophysics
Date Deposited: 22 Jun 2011 07:33
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