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Electrokinetics of miniature K+ channel: open-state V sensitivity and inhibition by K+ driving force.

Gazzarrini, Sabrina ; Abenavoli, Alessandra ; Gradmann, Dietrich ; Thiel, Gerhard ; Moroni, Anna (2006)
Electrokinetics of miniature K+ channel: open-state V sensitivity and inhibition by K+ driving force.
In: The Journal of membrane biology, 214 (1)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Kcv, isolated from a Chlorella virus, is the smallest known K+ channel. When Kcv is expressed in Xenopus oocytes and exposed to 50 mM: [K+](o), its open-state current-voltage relationship (I-V) has the shape of a "tilted S" between -200 and +120 mV. Details of this shape depend on the conditioning voltage (V (c)) immediately before an I-V recording. Unexpectedly, the I-V relationships, recorded in different [K+](o), do intersect. These characteristics are numerically described here by fits of a kinetic model to the experimental data. In this model, the V (c) sensitivity of I-V is mainly assigned to an affinity increase of external K+ association at more positive voltages. The general, tilted-S shape as well as the unexpected intersections of the I-V relationships are kinetically described by a decrease of the cord conductance by the electrochemical driving force for K+ in either direction, like in fast V-dependent blocking by competing ions.

Typ des Eintrags: Artikel
Erschienen: 2006
Autor(en): Gazzarrini, Sabrina ; Abenavoli, Alessandra ; Gradmann, Dietrich ; Thiel, Gerhard ; Moroni, Anna
Art des Eintrags: Bibliographie
Titel: Electrokinetics of miniature K+ channel: open-state V sensitivity and inhibition by K+ driving force.
Sprache: Englisch
Publikationsjahr: 2006
Titel der Zeitschrift, Zeitung oder Schriftenreihe: The Journal of membrane biology
Jahrgang/Volume einer Zeitschrift: 214
(Heft-)Nummer: 1
Kurzbeschreibung (Abstract):

Kcv, isolated from a Chlorella virus, is the smallest known K+ channel. When Kcv is expressed in Xenopus oocytes and exposed to 50 mM: [K+](o), its open-state current-voltage relationship (I-V) has the shape of a "tilted S" between -200 and +120 mV. Details of this shape depend on the conditioning voltage (V (c)) immediately before an I-V recording. Unexpectedly, the I-V relationships, recorded in different [K+](o), do intersect. These characteristics are numerically described here by fits of a kinetic model to the experimental data. In this model, the V (c) sensitivity of I-V is mainly assigned to an affinity increase of external K+ association at more positive voltages. The general, tilted-S shape as well as the unexpected intersections of the I-V relationships are kinetically described by a decrease of the cord conductance by the electrochemical driving force for K+ in either direction, like in fast V-dependent blocking by competing ions.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen)
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10 Fachbereich Biologie
Hinterlegungsdatum: 22 Jun 2011 07:22
Letzte Änderung: 05 Mär 2013 09:49
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