TU Darmstadt / ULB / TUbiblio

Chlorella viruses prevent multiple infections by depolarizing the host membrane.

Greiner, Timo and Frohns, Florian and Kang, Ming and Van Etten, James L. and Käsmann, Anja and Moroni, Anna and Hertel, Brigitte and Thiel, Gerhard (2009):
Chlorella viruses prevent multiple infections by depolarizing the host membrane.
In: The Journal of general virology, pp. 2033-9, 90, (Pt 8), ISSN 0022-1317,
[Article]

Abstract

Previous experiments established that when the unicellular green alga Chlorella NC64A is inoculated with two viruses, usually only one virus replicates in a single cell. That is, the viruses mutually exclude one another. In the current study, we explore the possibility that virus-induced host membrane depolarization, at least partially caused by a virus-encoded K(+) channel (Kcv), is involved in this mutual exclusion. Two chlorella viruses, PBCV-1 and NY-2A, were chosen for the study because (i) they can be distinguished by real-time PCR and (ii) they exhibit differential sensitivity to Cs(+), a well-known K(+) channel blocker. PBCV-1-induced host membrane depolarization, Kcv channel activity and plaque formation are only slightly affected by Cs(+), whereas all three NY-2A-induced events are strongly inhibited by Cs(+). The addition of one virus 5-15 min before the other results primarily in replication of the first virus. However, if virus NY-2A-induced membrane depolarization of the host is blocked by Cs(+), PBCV-1 is not excluded. We conclude that virus-induced membrane depolarization is at least partially responsible for the exclusion phenomenon.

Item Type: Article
Erschienen: 2009
Creators: Greiner, Timo and Frohns, Florian and Kang, Ming and Van Etten, James L. and Käsmann, Anja and Moroni, Anna and Hertel, Brigitte and Thiel, Gerhard
Title: Chlorella viruses prevent multiple infections by depolarizing the host membrane.
Language: English
Abstract:

Previous experiments established that when the unicellular green alga Chlorella NC64A is inoculated with two viruses, usually only one virus replicates in a single cell. That is, the viruses mutually exclude one another. In the current study, we explore the possibility that virus-induced host membrane depolarization, at least partially caused by a virus-encoded K(+) channel (Kcv), is involved in this mutual exclusion. Two chlorella viruses, PBCV-1 and NY-2A, were chosen for the study because (i) they can be distinguished by real-time PCR and (ii) they exhibit differential sensitivity to Cs(+), a well-known K(+) channel blocker. PBCV-1-induced host membrane depolarization, Kcv channel activity and plaque formation are only slightly affected by Cs(+), whereas all three NY-2A-induced events are strongly inhibited by Cs(+). The addition of one virus 5-15 min before the other results primarily in replication of the first virus. However, if virus NY-2A-induced membrane depolarization of the host is blocked by Cs(+), PBCV-1 is not excluded. We conclude that virus-induced membrane depolarization is at least partially responsible for the exclusion phenomenon.

Journal or Publication Title: The Journal of general virology
Volume: 90
Number: Pt 8
Divisions: 10 Department of Biology > Plant Membrane Biophysics
?? fb10_botanik ??
10 Department of Biology
Date Deposited: 21 Jun 2011 12:05
Export:
Suche nach Titel in: TUfind oder in Google

Optionen (nur für Redakteure)

View Item View Item