TU Darmstadt / ULB / TUbiblio

Distinct fluorescent pattern of KAT1::GFP in the plasma membrane of Vicia faba guard cells.

Homann, Ulrike ; Meckel, Tobias ; Hewing, Jennifer ; Hütt, Marc-Thorsten ; Hurst, Annette C. (2007)
Distinct fluorescent pattern of KAT1::GFP in the plasma membrane of Vicia faba guard cells.
In: European journal of cell biology, 86 (8)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

The organisation of membrane proteins into certain domains of the plasma membrane (PM) has been proposed to be important for signalling in yeast and animal cells. Here we describe the formation of a very distinct pattern of the K(+) channel KAT1 fused to the green fluorescent protein (KAT1::GFP) when transiently expressed in guard cells of Vicia faba. Using confocal laser scanning microscopy we observed a radially striped pattern of KAT1::GFP fluorescence in the PM in about 70% of all transfected guard cells. This characteristic pattern was found to be cell type and protein specific and independent of the stomatal aperture and the cytoskeleton. Staining of the cell wall of guard cells with Calcofluor White revealed a great similarity between the arrangement of cellulose microfibrils and the KAT1::GFP pattern. Furthermore, the radial pattern of KAT1::GFP immediately disappeared when turgor pressure was strongly decreased by changing from hypotonic to hypertonic conditions. The pattern reappeared within 15 min upon reestablishment of high turgor pressure in hypotonic solution. Evaluation of the staining pattern by a mathematical algorithm further confirmed this reversible abolishment of the radial pattern during hypertonic treatment. We therefore conclude that the radial organisation of KAT1::GFP depends on the close contact between the PM and cell wall in turgid guard cells. These results offer the first indication for a role of the cell wall in the localisation of ion channels. We propose a model in which KAT1 is located in the cellulose fibrils intermediate areas of the PM and discuss the physiological role of this phenomenon.

Typ des Eintrags: Artikel
Erschienen: 2007
Autor(en): Homann, Ulrike ; Meckel, Tobias ; Hewing, Jennifer ; Hütt, Marc-Thorsten ; Hurst, Annette C.
Art des Eintrags: Bibliographie
Titel: Distinct fluorescent pattern of KAT1::GFP in the plasma membrane of Vicia faba guard cells.
Sprache: Englisch
Publikationsjahr: 2007
Titel der Zeitschrift, Zeitung oder Schriftenreihe: European journal of cell biology
Jahrgang/Volume einer Zeitschrift: 86
(Heft-)Nummer: 8
Kurzbeschreibung (Abstract):

The organisation of membrane proteins into certain domains of the plasma membrane (PM) has been proposed to be important for signalling in yeast and animal cells. Here we describe the formation of a very distinct pattern of the K(+) channel KAT1 fused to the green fluorescent protein (KAT1::GFP) when transiently expressed in guard cells of Vicia faba. Using confocal laser scanning microscopy we observed a radially striped pattern of KAT1::GFP fluorescence in the PM in about 70% of all transfected guard cells. This characteristic pattern was found to be cell type and protein specific and independent of the stomatal aperture and the cytoskeleton. Staining of the cell wall of guard cells with Calcofluor White revealed a great similarity between the arrangement of cellulose microfibrils and the KAT1::GFP pattern. Furthermore, the radial pattern of KAT1::GFP immediately disappeared when turgor pressure was strongly decreased by changing from hypotonic to hypertonic conditions. The pattern reappeared within 15 min upon reestablishment of high turgor pressure in hypotonic solution. Evaluation of the staining pattern by a mathematical algorithm further confirmed this reversible abolishment of the radial pattern during hypertonic treatment. We therefore conclude that the radial organisation of KAT1::GFP depends on the close contact between the PM and cell wall in turgid guard cells. These results offer the first indication for a role of the cell wall in the localisation of ion channels. We propose a model in which KAT1 is located in the cellulose fibrils intermediate areas of the PM and discuss the physiological role of this phenomenon.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
?? fb10_botanik ??
10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen)
10 Fachbereich Biologie > Membrane Dynamics
10 Fachbereich Biologie > Pflanzliche Zellbiologie
Hinterlegungsdatum: 06 Jun 2011 13:38
Letzte Änderung: 05 Mär 2013 09:48
PPN:
Export:
Suche nach Titel in: TUfind oder in Google
Frage zum Eintrag Frage zum Eintrag

Optionen (nur für Redakteure)
Redaktionelle Details anzeigen Redaktionelle Details anzeigen