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Hydroquinone: O-glucosyltransferase from cultivated Rauvolfia cells: enrichment and partial amino acid sequences.

Arend, J. ; Warzecha, H. ; Stöckigt, J. :
Hydroquinone: O-glucosyltransferase from cultivated Rauvolfia cells: enrichment and partial amino acid sequences.
In: Phytochemistry, 53 (2) pp. 187-93. ISSN 0031-9422
[Artikel], (2000)

Kurzbeschreibung (Abstract)

Plant cell suspension cultures of Rauvolfia are able to produce a high amount of arbutin by glucosylation of exogenously added hydroquinone. A four step purification procedure using anion exchange, hydrophobic interaction, hydroxyapatite-chromatography and chromatofocusing delivered in a yield of 0.5%, an approximately 390 fold enrichment of the involved glucosyltransferase. SDS-PAGE showed a M(r) for the enzyme of 52 kDa. Proteolysis of the pure enzyme with endoproteinase LysC revealed six peptide fragments with 9-23 amino acids which were sequenced. Sequence alignment of the six peptides showed high homologies to glycosyltransferases from other higher plants.

Typ des Eintrags: Artikel
Erschienen: 2000
Autor(en): Arend, J. ; Warzecha, H. ; Stöckigt, J.
Titel: Hydroquinone: O-glucosyltransferase from cultivated Rauvolfia cells: enrichment and partial amino acid sequences.
Sprache: Englisch
Kurzbeschreibung (Abstract):

Plant cell suspension cultures of Rauvolfia are able to produce a high amount of arbutin by glucosylation of exogenously added hydroquinone. A four step purification procedure using anion exchange, hydrophobic interaction, hydroxyapatite-chromatography and chromatofocusing delivered in a yield of 0.5%, an approximately 390 fold enrichment of the involved glucosyltransferase. SDS-PAGE showed a M(r) for the enzyme of 52 kDa. Proteolysis of the pure enzyme with endoproteinase LysC revealed six peptide fragments with 9-23 amino acids which were sequenced. Sequence alignment of the six peptides showed high homologies to glycosyltransferases from other higher plants.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Phytochemistry
Band: 53
(Heft-)Nummer: 2
Fachbereich(e)/-gebiet(e): Fachbereich Biologie, Biology > Pflanzenbiotechnologie, Plant Biotechnology and Metabolic Engineering
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Fachbereich Biologie, Biology
Hinterlegungsdatum: 17 Mär 2011 12:28
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