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Two genes encoding gas vacuole proteins in Halobacterium halobium.

Horne, M. ; Englert, C. ; Pfeifer, Felicitas (1988)
Two genes encoding gas vacuole proteins in Halobacterium halobium.
In: Molecular & general genetics : MGG, 213 (2-3)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

The archaebacterium Halobacterium halobium contains two related gas vacuole protein-encoding genes (vac). One of these genes encodes a protein of 76 amino acids and resides on the major plasmid. The second gene is located on the chromosome in a (G + C)-rich DNA fraction and encodes a slightly larger but highly homologous protein consisting of 79 amino acids. The plasmid encoded vac gene is transcribed constitutively throughout the growth cycle while the chromosomal vac gene is expressed during the stationary phase of growth. Comparison of the nucleotide sequences of the two genes indicates differences in the putative promoter regions as well as 35 single base-pair exchanges within the coding regions of the two genes. The majority of the nucleotide exchanges in the coding region occur in the third position of a codon triplet generating the codon synonym. The only differences between the two encoded proteins are the exchange of 2 amino acids (positions 8 and 29) and a deletion of 3 amino acids near the carboxy-terminus of the plasmid encoded vac protein. The genomic DNAs from other halobacterial isolates (Halobacterium sp. SB3, GN101 and YC819-9) were found to contain only a chromosomal vac gene copy. There is a high conservation of the chromosomal vac gene and the genomic region surrounding it among the halobacterial strains investigated.

Typ des Eintrags: Artikel
Erschienen: 1988
Autor(en): Horne, M. ; Englert, C. ; Pfeifer, Felicitas
Art des Eintrags: Bibliographie
Titel: Two genes encoding gas vacuole proteins in Halobacterium halobium.
Sprache: Englisch
Publikationsjahr: 1988
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Molecular & general genetics : MGG
Jahrgang/Volume einer Zeitschrift: 213
(Heft-)Nummer: 2-3
Kurzbeschreibung (Abstract):

The archaebacterium Halobacterium halobium contains two related gas vacuole protein-encoding genes (vac). One of these genes encodes a protein of 76 amino acids and resides on the major plasmid. The second gene is located on the chromosome in a (G + C)-rich DNA fraction and encodes a slightly larger but highly homologous protein consisting of 79 amino acids. The plasmid encoded vac gene is transcribed constitutively throughout the growth cycle while the chromosomal vac gene is expressed during the stationary phase of growth. Comparison of the nucleotide sequences of the two genes indicates differences in the putative promoter regions as well as 35 single base-pair exchanges within the coding regions of the two genes. The majority of the nucleotide exchanges in the coding region occur in the third position of a codon triplet generating the codon synonym. The only differences between the two encoded proteins are the exchange of 2 amino acids (positions 8 and 29) and a deletion of 3 amino acids near the carboxy-terminus of the plasmid encoded vac protein. The genomic DNAs from other halobacterial isolates (Halobacterium sp. SB3, GN101 and YC819-9) were found to contain only a chromosomal vac gene copy. There is a high conservation of the chromosomal vac gene and the genomic region surrounding it among the halobacterial strains investigated.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie > Microbiology and Archaea
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10 Fachbereich Biologie
Hinterlegungsdatum: 14 Feb 2011 13:12
Letzte Änderung: 05 Mär 2013 09:45
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