Pfeifer, Felicitas ; Blaseio, U. ; Horne, M. (1989)
Genome structure of Halobacterium halobium: plasmid dynamics in gas vacuole deficient mutants.
In: Canadian journal of microbiology, 35 (1)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stationary phase of growth is mutated with a frequency of 10(-5). The difference in the mutation susceptibility is due to the dynamics of plasmid pHH1. p-vac gene mutations are caused (i) by the integration of an insertion element or (ii) by a deletion event encompassing the p-vac gene region. In contrast, c-vac mutants analyzed to date incurred neither insertion elements nor deletions. Deletion events within pHH1 occur at high frequencies during the development of a H. halobium culture. The investigation of the fusion regions resulting from deletion events indicates that insertion elements are involved. The analysis of pHH1 deletion variants led to a 4 kilobase pair DNA region containing the origin of replication of the pHH1 plasmid.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1989 |
| Autor(en): | Pfeifer, Felicitas ; Blaseio, U. ; Horne, M. |
| Art des Eintrags: | Bibliographie |
| Titel: | Genome structure of Halobacterium halobium: plasmid dynamics in gas vacuole deficient mutants. |
| Sprache: | Englisch |
| Publikationsjahr: | 1989 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Canadian journal of microbiology |
| Jahrgang/Volume einer Zeitschrift: | 35 |
| (Heft-)Nummer: | 1 |
| Kurzbeschreibung (Abstract): | Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stationary phase of growth is mutated with a frequency of 10(-5). The difference in the mutation susceptibility is due to the dynamics of plasmid pHH1. p-vac gene mutations are caused (i) by the integration of an insertion element or (ii) by a deletion event encompassing the p-vac gene region. In contrast, c-vac mutants analyzed to date incurred neither insertion elements nor deletions. Deletion events within pHH1 occur at high frequencies during the development of a H. halobium culture. The investigation of the fusion regions resulting from deletion events indicates that insertion elements are involved. The analysis of pHH1 deletion variants led to a 4 kilobase pair DNA region containing the origin of replication of the pHH1 plasmid. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Microbiology and Archaea ?? fb10_mikrobiologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 14 Feb 2011 11:02 |
| Letzte Änderung: | 05 Mär 2013 09:45 |
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