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Genome structure of Halobacterium halobium: plasmid dynamics in gas vacuole deficient mutants.

Pfeifer, F. and Blaseio, U. and Horne, M. :
Genome structure of Halobacterium halobium: plasmid dynamics in gas vacuole deficient mutants.
In: Canadian journal of microbiology, 35 (1) pp. 96-100. ISSN 0008-4166
[Article] , (1989)

Abstract

Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stationary phase of growth is mutated with a frequency of 10(-5). The difference in the mutation susceptibility is due to the dynamics of plasmid pHH1. p-vac gene mutations are caused (i) by the integration of an insertion element or (ii) by a deletion event encompassing the p-vac gene region. In contrast, c-vac mutants analyzed to date incurred neither insertion elements nor deletions. Deletion events within pHH1 occur at high frequencies during the development of a H. halobium culture. The investigation of the fusion regions resulting from deletion events indicates that insertion elements are involved. The analysis of pHH1 deletion variants led to a 4 kilobase pair DNA region containing the origin of replication of the pHH1 plasmid.

Item Type: Article
Erschienen: 1989
Creators: Pfeifer, F. and Blaseio, U. and Horne, M.
Title: Genome structure of Halobacterium halobium: plasmid dynamics in gas vacuole deficient mutants.
Language: English
Abstract:

Halobacterium halobium contains two gas vacuole protein genes that are located in plasmid pHH1 (p-vac) and in the chromosomal DNA (c-vac). The mutation frequency for these genes is different: the constitutively expressed p-vac gene is mutated with a frequency of 10(-2), while the chromosomal gene expressed in the stationary phase of growth is mutated with a frequency of 10(-5). The difference in the mutation susceptibility is due to the dynamics of plasmid pHH1. p-vac gene mutations are caused (i) by the integration of an insertion element or (ii) by a deletion event encompassing the p-vac gene region. In contrast, c-vac mutants analyzed to date incurred neither insertion elements nor deletions. Deletion events within pHH1 occur at high frequencies during the development of a H. halobium culture. The investigation of the fusion regions resulting from deletion events indicates that insertion elements are involved. The analysis of pHH1 deletion variants led to a 4 kilobase pair DNA region containing the origin of replication of the pHH1 plasmid.

Journal or Publication Title: Canadian journal of microbiology
Volume: 35
Number: 1
Divisions: 10 Department of Biology > Microbiology and Archaea
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10 Department of Biology
Date Deposited: 14 Feb 2011 11:02
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