TU Darmstadt / ULB / TUbiblio

Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels.

Bertl, A. and Anderson, J. A. and Slayman, C. L. and Sentenac, H. and Gaber, R. F. (1994):
Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels.
In: Folia microbiologica, pp. 507-9, 39, (6), ISSN 0015-5632,
[Online-Edition: http://www.springerlink.com/content/21w7448413778m3n/fulltex...],
[Article]

Abstract

Disruption of genes encoding endogenous transport proteins in Saccharomyces cerevisiae has facilitated the recent cloning, by functional expression, of cDNAs encoding K+ channels and amino acid transporters from the plant Arabidopsis thaliana [1-4]. In the present study, we demonstrate in whole-cell patch clamp experiments that the inability of trk1deltatrk2delta mutants of S. cerevisiae to grow on submillimolar K+ correlates with the lack of K+ inward currents, which are present in wild-type cells, and that transformation of the trk1deltatrk2delta double-deletion mutant with KAT1 from Arabidopsis thaliana restores this phenotype by encoding a plasma membrane protein that allows large K+ inward currents. Similar K+ inward currents are induced by transformation of a trk1 mutant with AKT1 from A. thaliana.

Item Type: Article
Erschienen: 1994
Creators: Bertl, A. and Anderson, J. A. and Slayman, C. L. and Sentenac, H. and Gaber, R. F.
Title: Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels.
Language: English
Abstract:

Disruption of genes encoding endogenous transport proteins in Saccharomyces cerevisiae has facilitated the recent cloning, by functional expression, of cDNAs encoding K+ channels and amino acid transporters from the plant Arabidopsis thaliana [1-4]. In the present study, we demonstrate in whole-cell patch clamp experiments that the inability of trk1deltatrk2delta mutants of S. cerevisiae to grow on submillimolar K+ correlates with the lack of K+ inward currents, which are present in wild-type cells, and that transformation of the trk1deltatrk2delta double-deletion mutant with KAT1 from Arabidopsis thaliana restores this phenotype by encoding a plasma membrane protein that allows large K+ inward currents. Similar K+ inward currents are induced by transformation of a trk1 mutant with AKT1 from A. thaliana.

Journal or Publication Title: Folia microbiologica
Volume: 39
Number: 6
Divisions: 10 Department of Biology
?? fb10_botanik ??
10 Department of Biology > Yeast Membrane Biology
Date Deposited: 25 Nov 2010 15:29
Official URL: http://www.springerlink.com/content/21w7448413778m3n/fulltex...
Identification Number: doi:10.1007/BF02814074
Export:
Suche nach Titel in: TUfind oder in Google

Optionen (nur für Redakteure)

View Item View Item