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Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels.

Bertl, A. ; Anderson, J. A. ; Slayman, C. L. ; Sentenac, H. ; Gaber, R. F. (1994)
Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels.
In: Folia microbiologica, 39 (6)
doi: 10.1007/BF02814074
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Disruption of genes encoding endogenous transport proteins in Saccharomyces cerevisiae has facilitated the recent cloning, by functional expression, of cDNAs encoding K+ channels and amino acid transporters from the plant Arabidopsis thaliana [1-4]. In the present study, we demonstrate in whole-cell patch clamp experiments that the inability of trk1deltatrk2delta mutants of S. cerevisiae to grow on submillimolar K+ correlates with the lack of K+ inward currents, which are present in wild-type cells, and that transformation of the trk1deltatrk2delta double-deletion mutant with KAT1 from Arabidopsis thaliana restores this phenotype by encoding a plasma membrane protein that allows large K+ inward currents. Similar K+ inward currents are induced by transformation of a trk1 mutant with AKT1 from A. thaliana.

Typ des Eintrags: Artikel
Erschienen: 1994
Autor(en): Bertl, A. ; Anderson, J. A. ; Slayman, C. L. ; Sentenac, H. ; Gaber, R. F.
Art des Eintrags: Bibliographie
Titel: Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels.
Sprache: Englisch
Publikationsjahr: 1994
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Folia microbiologica
Jahrgang/Volume einer Zeitschrift: 39
(Heft-)Nummer: 6
DOI: 10.1007/BF02814074
Kurzbeschreibung (Abstract):

Disruption of genes encoding endogenous transport proteins in Saccharomyces cerevisiae has facilitated the recent cloning, by functional expression, of cDNAs encoding K+ channels and amino acid transporters from the plant Arabidopsis thaliana [1-4]. In the present study, we demonstrate in whole-cell patch clamp experiments that the inability of trk1deltatrk2delta mutants of S. cerevisiae to grow on submillimolar K+ correlates with the lack of K+ inward currents, which are present in wild-type cells, and that transformation of the trk1deltatrk2delta double-deletion mutant with KAT1 from Arabidopsis thaliana restores this phenotype by encoding a plasma membrane protein that allows large K+ inward currents. Similar K+ inward currents are induced by transformation of a trk1 mutant with AKT1 from A. thaliana.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
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10 Fachbereich Biologie > Yeast Membrane Biology
Hinterlegungsdatum: 25 Nov 2010 15:29
Letzte Änderung: 09 Mär 2022 12:51
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