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Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels.

Bertl, A. ; Anderson, J. A. ; Slayman, C. L. ; Sentenac, H. ; Gaber, R. F. :
Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels.
[Online-Edition: http://www.springerlink.com/content/21w7448413778m3n/fulltex...]
In: Folia microbiologica, 39 (6) pp. 507-9. ISSN 0015-5632
[Artikel], (1994)

Offizielle URL: http://www.springerlink.com/content/21w7448413778m3n/fulltex...

Kurzbeschreibung (Abstract)

Disruption of genes encoding endogenous transport proteins in Saccharomyces cerevisiae has facilitated the recent cloning, by functional expression, of cDNAs encoding K+ channels and amino acid transporters from the plant Arabidopsis thaliana [1-4]. In the present study, we demonstrate in whole-cell patch clamp experiments that the inability of trk1deltatrk2delta mutants of S. cerevisiae to grow on submillimolar K+ correlates with the lack of K+ inward currents, which are present in wild-type cells, and that transformation of the trk1deltatrk2delta double-deletion mutant with KAT1 from Arabidopsis thaliana restores this phenotype by encoding a plasma membrane protein that allows large K+ inward currents. Similar K+ inward currents are induced by transformation of a trk1 mutant with AKT1 from A. thaliana.

Typ des Eintrags: Artikel
Erschienen: 1994
Autor(en): Bertl, A. ; Anderson, J. A. ; Slayman, C. L. ; Sentenac, H. ; Gaber, R. F.
Titel: Inward and outward rectifying potassium currents in Saccharomyces cerevisiae mediated by endogenous and heterelogously expressed ion channels.
Sprache: Englisch
Kurzbeschreibung (Abstract):

Disruption of genes encoding endogenous transport proteins in Saccharomyces cerevisiae has facilitated the recent cloning, by functional expression, of cDNAs encoding K+ channels and amino acid transporters from the plant Arabidopsis thaliana [1-4]. In the present study, we demonstrate in whole-cell patch clamp experiments that the inability of trk1deltatrk2delta mutants of S. cerevisiae to grow on submillimolar K+ correlates with the lack of K+ inward currents, which are present in wild-type cells, and that transformation of the trk1deltatrk2delta double-deletion mutant with KAT1 from Arabidopsis thaliana restores this phenotype by encoding a plasma membrane protein that allows large K+ inward currents. Similar K+ inward currents are induced by transformation of a trk1 mutant with AKT1 from A. thaliana.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Folia microbiologica
Band: 39
(Heft-)Nummer: 6
Fachbereich(e)/-gebiet(e): Fachbereich Biologie, Biology
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Fachbereich Biologie, Biology > Hefe-Membranbiologie, Yeast Membrane Biology
Hinterlegungsdatum: 25 Nov 2010 15:29
Offizielle URL: http://www.springerlink.com/content/21w7448413778m3n/fulltex...
ID-Nummer: 10.1007/BF02814074
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