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Assembly of plant Shaker-like K(out) channels requires two distinct sites of the channel alpha-subunit.

Dreyer, I. ; Porée, F. ; Schneider, A. ; Mittelstädt, J. ; Bertl, A. ; Sentenac, H. ; Thibaud, J. ; Mueller-Roeber, B. (2004)
Assembly of plant Shaker-like K(out) channels requires two distinct sites of the channel alpha-subunit.
In: Biophysical journal, 87 (2)
doi: 10.1529/biophysj.103.037671
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

SKOR and GORK are outward-rectifying plant potassium channels from Arabidopsis thaliana. They belong to the Shaker superfamily of voltage-dependent K(+) channels. Channels of this class are composed of four alpha-subunits and subunit assembly is a prerequisite for channel function. In this study the assembly mechanism of SKOR was investigated using the yeast two-hybrid system and functional assays in Xenopus oocytes and in yeast. We demonstrate that SKOR and GORK physically interact and assemble into heteromeric K(out) channels. Deletion mutants and chimeric proteins generated from SKOR and the K(in) channel alpha-subunit KAT1 revealed that the cytoplasmic C-terminus of SKOR determines channel assembly. Two domains that are crucial for channel assembly were identified: i), a proximal interacting region comprising a putative cyclic nucleotide-binding domain together with 33 amino acids just upstream of this domain, and ii), a distal interacting region showing some resemblance to the K(T) domain of KAT1. Both regions contributed differently to channel assembly. Whereas the proximal interacting region was found to be active on its own, the distal interacting region required an intact proximal interacting region to be active. K(out) alpha-subunits did not assemble with K(in) alpha-subunits because of the absence of interaction between their assembly sites.

Typ des Eintrags: Artikel
Erschienen: 2004
Autor(en): Dreyer, I. ; Porée, F. ; Schneider, A. ; Mittelstädt, J. ; Bertl, A. ; Sentenac, H. ; Thibaud, J. ; Mueller-Roeber, B.
Art des Eintrags: Bibliographie
Titel: Assembly of plant Shaker-like K(out) channels requires two distinct sites of the channel alpha-subunit.
Sprache: Englisch
Publikationsjahr: 2004
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Biophysical journal
Jahrgang/Volume einer Zeitschrift: 87
(Heft-)Nummer: 2
DOI: 10.1529/biophysj.103.037671
Kurzbeschreibung (Abstract):

SKOR and GORK are outward-rectifying plant potassium channels from Arabidopsis thaliana. They belong to the Shaker superfamily of voltage-dependent K(+) channels. Channels of this class are composed of four alpha-subunits and subunit assembly is a prerequisite for channel function. In this study the assembly mechanism of SKOR was investigated using the yeast two-hybrid system and functional assays in Xenopus oocytes and in yeast. We demonstrate that SKOR and GORK physically interact and assemble into heteromeric K(out) channels. Deletion mutants and chimeric proteins generated from SKOR and the K(in) channel alpha-subunit KAT1 revealed that the cytoplasmic C-terminus of SKOR determines channel assembly. Two domains that are crucial for channel assembly were identified: i), a proximal interacting region comprising a putative cyclic nucleotide-binding domain together with 33 amino acids just upstream of this domain, and ii), a distal interacting region showing some resemblance to the K(T) domain of KAT1. Both regions contributed differently to channel assembly. Whereas the proximal interacting region was found to be active on its own, the distal interacting region required an intact proximal interacting region to be active. K(out) alpha-subunits did not assemble with K(in) alpha-subunits because of the absence of interaction between their assembly sites.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
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10 Fachbereich Biologie > Yeast Membrane Biology
Hinterlegungsdatum: 29 Nov 2010 15:26
Letzte Änderung: 05 Mär 2013 09:41
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