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Plant K(in) and K(out) channels: approaching the trait of opposite rectification by analyzing more than 250 KAT1-SKOR chimeras.

Porée, F. and Wulfetange, K. and Naso, A. and Carpaneto, A. and Roller, A. and Natura, G. and Bertl, A. and Sentenac, H. and Thibaud, J. and Dreyer, I. (2005):
Plant K(in) and K(out) channels: approaching the trait of opposite rectification by analyzing more than 250 KAT1-SKOR chimeras.
In: Biochemical and biophysical research communications, pp. 465-73, 332, (2), ISSN 0006-291X,
[Online-Edition: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WB...],
[Article]

Abstract

Members of the Shaker-like plant K(+) channel family share a common structure, but are highly diverse in their function: they behave as either hyperpolarization-activated inward-rectifying (K(in)) channels, or leak-like (K(weak)) channels, or depolarization-activated outward-rectifying (K(out)) channels. Here we created 256 chimeras between the K(in) channel KAT1 and the K(out) channel SKOR. The chimeras were screened in a potassium-uptake deficient yeast strain to identify those, which mediate potassium inward currents, i.e., which are functionally equivalent to KAT1. This strategy allowed us to identify three chimeras which differ from KAT1 in three parts of the polypeptide: the cytosolic N-terminus, the cytosolic C-terminus, and the putative voltage-sensor S4. Additionally, mutations in the K(out) channel SKOR were generated in order to localize molecular entities underlying its depolarization activation. The triple mutant SKOR-D312N-M313L-I314G, carrying amino-acid changes in the S6 segment, was identified as a channel which did not display any rectification in the tested voltage-range.

Item Type: Article
Erschienen: 2005
Creators: Porée, F. and Wulfetange, K. and Naso, A. and Carpaneto, A. and Roller, A. and Natura, G. and Bertl, A. and Sentenac, H. and Thibaud, J. and Dreyer, I.
Title: Plant K(in) and K(out) channels: approaching the trait of opposite rectification by analyzing more than 250 KAT1-SKOR chimeras.
Language: English
Abstract:

Members of the Shaker-like plant K(+) channel family share a common structure, but are highly diverse in their function: they behave as either hyperpolarization-activated inward-rectifying (K(in)) channels, or leak-like (K(weak)) channels, or depolarization-activated outward-rectifying (K(out)) channels. Here we created 256 chimeras between the K(in) channel KAT1 and the K(out) channel SKOR. The chimeras were screened in a potassium-uptake deficient yeast strain to identify those, which mediate potassium inward currents, i.e., which are functionally equivalent to KAT1. This strategy allowed us to identify three chimeras which differ from KAT1 in three parts of the polypeptide: the cytosolic N-terminus, the cytosolic C-terminus, and the putative voltage-sensor S4. Additionally, mutations in the K(out) channel SKOR were generated in order to localize molecular entities underlying its depolarization activation. The triple mutant SKOR-D312N-M313L-I314G, carrying amino-acid changes in the S6 segment, was identified as a channel which did not display any rectification in the tested voltage-range.

Journal or Publication Title: Biochemical and biophysical research communications
Volume: 332
Number: 2
Divisions: 10 Department of Biology
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10 Department of Biology > Yeast Membrane Biology
Date Deposited: 29 Nov 2010 15:29
Official URL: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WB...
Identification Number: doi:10.1016/j.bbrc.2005.04.150
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