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From sequence to antibody: genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel.

Gehwolf, R. ; Weiss, R. ; Gabler, M. ; Hurst, A. C. ; Bertl, A. ; Thalhamer, J. ; Obermeyer, G. (2007)
From sequence to antibody: genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel.
In: FEBS letters, 581 (3)
doi: 10.1016/j.febslet.2007.01.004
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Monoclonal antibodies against the K(+) channel KAT1 of Arabidopsis thaliana, a low abundance, plant plasma membrane protein, were generated by genetic immunisation to avoid the time and labour consuming purification of native or recombinant proteins and peptides usually necessary for conventional immunisation techniques. The resulting polyclonal and monoclonal antibody sera recognised a single protein band in a microsomal fraction of wild-type A. thaliana leaves and in membrane fractions of transgenic yeast cells and tobacco plants expressing the KAT1 protein. Therefore, genetic immunisation is suitable for generating monoclonal antibodies against plant proteins and particularly, against plant membrane proteins of low abundance.

Typ des Eintrags: Artikel
Erschienen: 2007
Autor(en): Gehwolf, R. ; Weiss, R. ; Gabler, M. ; Hurst, A. C. ; Bertl, A. ; Thalhamer, J. ; Obermeyer, G.
Art des Eintrags: Bibliographie
Titel: From sequence to antibody: genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel.
Sprache: Englisch
Publikationsjahr: 2007
Titel der Zeitschrift, Zeitung oder Schriftenreihe: FEBS letters
Jahrgang/Volume einer Zeitschrift: 581
(Heft-)Nummer: 3
DOI: 10.1016/j.febslet.2007.01.004
Kurzbeschreibung (Abstract):

Monoclonal antibodies against the K(+) channel KAT1 of Arabidopsis thaliana, a low abundance, plant plasma membrane protein, were generated by genetic immunisation to avoid the time and labour consuming purification of native or recombinant proteins and peptides usually necessary for conventional immunisation techniques. The resulting polyclonal and monoclonal antibody sera recognised a single protein band in a microsomal fraction of wild-type A. thaliana leaves and in membrane fractions of transgenic yeast cells and tobacco plants expressing the KAT1 protein. Therefore, genetic immunisation is suitable for generating monoclonal antibodies against plant proteins and particularly, against plant membrane proteins of low abundance.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
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10 Fachbereich Biologie > Yeast Membrane Biology
Hinterlegungsdatum: 29 Nov 2010 15:31
Letzte Änderung: 05 Mär 2013 09:41
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