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From sequence to antibody: genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel.

Gehwolf, R. and Weiss, R. and Gabler, M. and Hurst, A. C. and Bertl, A. and Thalhamer, J. and Obermeyer, G. (2007):
From sequence to antibody: genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel.
In: FEBS letters, pp. 448-52, 581, (3), ISSN 0014-5793, [Online-Edition: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T3...],
[Article]

Abstract

Monoclonal antibodies against the K(+) channel KAT1 of Arabidopsis thaliana, a low abundance, plant plasma membrane protein, were generated by genetic immunisation to avoid the time and labour consuming purification of native or recombinant proteins and peptides usually necessary for conventional immunisation techniques. The resulting polyclonal and monoclonal antibody sera recognised a single protein band in a microsomal fraction of wild-type A. thaliana leaves and in membrane fractions of transgenic yeast cells and tobacco plants expressing the KAT1 protein. Therefore, genetic immunisation is suitable for generating monoclonal antibodies against plant proteins and particularly, against plant membrane proteins of low abundance.

Item Type: Article
Erschienen: 2007
Creators: Gehwolf, R. and Weiss, R. and Gabler, M. and Hurst, A. C. and Bertl, A. and Thalhamer, J. and Obermeyer, G.
Title: From sequence to antibody: genetic immunisation is suitable to generate antibodies against a rare plant membrane protein, the KAT 1 channel.
Language: English
Abstract:

Monoclonal antibodies against the K(+) channel KAT1 of Arabidopsis thaliana, a low abundance, plant plasma membrane protein, were generated by genetic immunisation to avoid the time and labour consuming purification of native or recombinant proteins and peptides usually necessary for conventional immunisation techniques. The resulting polyclonal and monoclonal antibody sera recognised a single protein band in a microsomal fraction of wild-type A. thaliana leaves and in membrane fractions of transgenic yeast cells and tobacco plants expressing the KAT1 protein. Therefore, genetic immunisation is suitable for generating monoclonal antibodies against plant proteins and particularly, against plant membrane proteins of low abundance.

Journal or Publication Title: FEBS letters
Volume: 581
Number: 3
Divisions: 10 Department of Biology
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10 Department of Biology > Yeast Membrane Biology
Date Deposited: 29 Nov 2010 15:31
Official URL: http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T3...
Identification Number: doi:10.1016/j.febslet.2007.01.004
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