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A database search for hammerhead ribozyme motifs.

Gräf, S. and Przybilski, R. and Steger, G. and Hammann, Christian (2005):
A database search for hammerhead ribozyme motifs.
In: Biochemical Society transactions, pp. 477-8, 33, (Pt 3), ISSN 0300-5127, [Article]

Abstract

The hammerhead ribozyme is the smallest naturally occurring RNA endonuclease. It is found in subviral plant pathogens and transcripts of satellite DNA from a limited number of organisms. We have performed a database search for novel examples of this catalytic RNA, taking into consideration the recently defined structural requirements for an efficient cleavage under physiological magnesium ion concentrations. In this search, we find, apart from the known examples, several hundreds of motifs in organisms of all kingdoms of life. In a first set of experiments, we analysed hammerhead ribozymes from Arabidopsis thaliana. We found that these sequences are tissue-specifically expressed and that they undergo self-cleavage in planta. Furthermore, their activity under physiological magnesium ion concentrations depends on functional loop-loop interactions, as shown by the lack of activity of appropriate mutants.

Item Type: Article
Erschienen: 2005
Creators: Gräf, S. and Przybilski, R. and Steger, G. and Hammann, Christian
Title: A database search for hammerhead ribozyme motifs.
Language: English
Abstract:

The hammerhead ribozyme is the smallest naturally occurring RNA endonuclease. It is found in subviral plant pathogens and transcripts of satellite DNA from a limited number of organisms. We have performed a database search for novel examples of this catalytic RNA, taking into consideration the recently defined structural requirements for an efficient cleavage under physiological magnesium ion concentrations. In this search, we find, apart from the known examples, several hundreds of motifs in organisms of all kingdoms of life. In a first set of experiments, we analysed hammerhead ribozymes from Arabidopsis thaliana. We found that these sequences are tissue-specifically expressed and that they undergo self-cleavage in planta. Furthermore, their activity under physiological magnesium ion concentrations depends on functional loop-loop interactions, as shown by the lack of activity of appropriate mutants.

Journal or Publication Title: Biochemical Society transactions
Volume: 33
Number: Pt 3
Divisions: 10 Department of Biology
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10 Department of Biology > Ribogenetics
Date Deposited: 29 Jul 2010 12:24
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