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Analysis of a protein region involved in permeation and gating of the voltage-gated Torpedo chloride channel ClC-0

Ludewig, U. ; Jentsch, T. J. ; Pusch, M. (1997)
Analysis of a protein region involved in permeation and gating of the voltage-gated Torpedo chloride channel ClC-0.
In: The Journal of physiology, 498 (3)
doi: 10.1113/jphysiol.1997.sp021893
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

1. The chloride channel from the Torpedo electric organ, ClC-0, is controlled by two distinct ('fast' and 'slow') voltage-dependent gates. Here we investigate the effects of mutations in a region after putative transmembrane domain D12. A mutation in this region has previously been shown to change fast gating and permeation. 2. We used a combination of site-directed mutagenesis with two-electrode voltage-clamp and patch-clamp measurements. 3. Most conservative substitutions have minor effects, while more drastic mutations change kinetics and voltage dependence of fast gating, as well as ion selectivity and rectification. 4. While ClC-0 wild-type (WT) channels deactivate fully in two-electrode voltage clamp at negative voltages, channels do not close completely in patch-clamp experiments. Open probability is increased by intracellular chloride in a concentration- but not voltage-dependent manner. 5. In several mutants, including K519R, the minimal macroscopic open probability of fast gating is larger than in WT. Mutant channels fluctuate at negative potentials between open and closed conformations. Open probability is much more effectively increased by intracellular chloride than in WT. The observations support the idea that permeating ions inside the pore stabilize the open state. 6. Besides effects on permeation and gating of single protopores, some mutations affect 'slow' gating. In summary, the region after D12 participates in fast as well as in slow gating; mutations additionally influence permeation properties.

Typ des Eintrags: Artikel
Erschienen: 1997
Autor(en): Ludewig, U. ; Jentsch, T. J. ; Pusch, M.
Art des Eintrags: Bibliographie
Titel: Analysis of a protein region involved in permeation and gating of the voltage-gated Torpedo chloride channel ClC-0
Sprache: Englisch
Publikationsjahr: 1 Februar 1997
Verlag: Wiley-Blackwell - STM
Titel der Zeitschrift, Zeitung oder Schriftenreihe: The Journal of physiology
Jahrgang/Volume einer Zeitschrift: 498
(Heft-)Nummer: 3
DOI: 10.1113/jphysiol.1997.sp021893
Kurzbeschreibung (Abstract):

1. The chloride channel from the Torpedo electric organ, ClC-0, is controlled by two distinct ('fast' and 'slow') voltage-dependent gates. Here we investigate the effects of mutations in a region after putative transmembrane domain D12. A mutation in this region has previously been shown to change fast gating and permeation. 2. We used a combination of site-directed mutagenesis with two-electrode voltage-clamp and patch-clamp measurements. 3. Most conservative substitutions have minor effects, while more drastic mutations change kinetics and voltage dependence of fast gating, as well as ion selectivity and rectification. 4. While ClC-0 wild-type (WT) channels deactivate fully in two-electrode voltage clamp at negative voltages, channels do not close completely in patch-clamp experiments. Open probability is increased by intracellular chloride in a concentration- but not voltage-dependent manner. 5. In several mutants, including K519R, the minimal macroscopic open probability of fast gating is larger than in WT. Mutant channels fluctuate at negative potentials between open and closed conformations. Open probability is much more effectively increased by intracellular chloride than in WT. The observations support the idea that permeating ions inside the pore stabilize the open state. 6. Besides effects on permeation and gating of single protopores, some mutations affect 'slow' gating. In summary, the region after D12 participates in fast as well as in slow gating; mutations additionally influence permeation properties.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
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10 Fachbereich Biologie > Pflanzenernährung und Biomasse
Hinterlegungsdatum: 16 Mär 2010 15:13
Letzte Änderung: 06 Apr 2020 12:39
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