Desimone, Marcelo ; Catoni, Elisabetta ; Ludewig, Uwe ; Hilpert, Melanie ; Schneider, Anja ; Kunze, Reinhard ; Tegeder, Mechthild ; Frommer, Wolf Bernd ; Schumacher, Karin (2002)
A novel superfamily of transporters for allantoin and other oxo derivatives of nitrogen heterocyclic compounds in Arabidopsis.
In: The Plant cell, 14 (4)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
A wide spectrum of soil heterocyclic nitrogen compounds are potential nutrients for plants. Here, it is shown that Arabidopsis plants are able to use allantoin as sole nitrogen source. By functional complementation of a yeast mutant defective in allantoin uptake, an Arabidopsis transporter, AtUPS1 (Arabidopsis thaliana ureide permease 1), was identified. AtUPS1 belongs to a novel superfamily of plant membrane proteins with five open reading frames in Arabidopsis (identity, 64 to 82%). UPS proteins have 10 putative transmembrane domains with a large cytosolic central domain containing a "Walker A" motif. Transport of (14)C-labeled allantoin by AtUPS1 in yeast exhibited saturation kinetics (K(m) approximately 52 microM), was dependent on Glc and a proton gradient, and was stimulated by acidic pH. AtUPS1 transports uric acid and xanthine, besides allantoin, but not adenine. Protons are cosubstrates in allantoin transport by AtUPS1, as demonstrated by expression in Xenopus laevis oocytes. In plants, AtUPS1 gene expression was dependent on the nitrogen source. Therefore, AtUPS1 presumably is involved in the uptake of allantoin and other purine degradation products when primary sources are limiting.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2002 |
| Autor(en): | Desimone, Marcelo ; Catoni, Elisabetta ; Ludewig, Uwe ; Hilpert, Melanie ; Schneider, Anja ; Kunze, Reinhard ; Tegeder, Mechthild ; Frommer, Wolf Bernd ; Schumacher, Karin |
| Art des Eintrags: | Bibliographie |
| Titel: | A novel superfamily of transporters for allantoin and other oxo derivatives of nitrogen heterocyclic compounds in Arabidopsis. |
| Sprache: | Englisch |
| Publikationsjahr: | 2002 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | The Plant cell |
| Jahrgang/Volume einer Zeitschrift: | 14 |
| (Heft-)Nummer: | 4 |
| Kurzbeschreibung (Abstract): | A wide spectrum of soil heterocyclic nitrogen compounds are potential nutrients for plants. Here, it is shown that Arabidopsis plants are able to use allantoin as sole nitrogen source. By functional complementation of a yeast mutant defective in allantoin uptake, an Arabidopsis transporter, AtUPS1 (Arabidopsis thaliana ureide permease 1), was identified. AtUPS1 belongs to a novel superfamily of plant membrane proteins with five open reading frames in Arabidopsis (identity, 64 to 82%). UPS proteins have 10 putative transmembrane domains with a large cytosolic central domain containing a "Walker A" motif. Transport of (14)C-labeled allantoin by AtUPS1 in yeast exhibited saturation kinetics (K(m) approximately 52 microM), was dependent on Glc and a proton gradient, and was stimulated by acidic pH. AtUPS1 transports uric acid and xanthine, besides allantoin, but not adenine. Protons are cosubstrates in allantoin transport by AtUPS1, as demonstrated by expression in Xenopus laevis oocytes. In plants, AtUPS1 gene expression was dependent on the nitrogen source. Therefore, AtUPS1 presumably is involved in the uptake of allantoin and other purine degradation products when primary sources are limiting. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Pflanzenernährung und Biomasse ?? fb10_botanik ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 17 Mär 2010 15:42 |
| Letzte Änderung: | 05 Mär 2013 09:32 |
| PPN: | |
| Export: | |
| Suche nach Titel in: | TUfind oder in Google |
 |
Frage zum Eintrag |
Optionen (nur für Redakteure)
 |
Redaktionelle Details anzeigen |
Drucken
Impressum