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Molecular determinants of ammonia and urea conductance in plant aquaporin homologs.

Dynowski, Marek and Mayer, Maria and Moran, Oscar and Ludewig, Uwe :
Molecular determinants of ammonia and urea conductance in plant aquaporin homologs.
In: FEBS letters, 582 (16) pp. 2458-62. ISSN 0014-5793
[Article] , (2008)

Abstract

Aquaporins and/or aquaglyceroporins regulate the permeability of plant membranes to water and small, uncharged molecules. Using molecular simulations with a plant plasma membrane aquaporin tetramer, the residues in the channel constriction region were identified as the crucial determinants of ammonia and urea conductance. The impact of these residues was experimentally verified using AtPIP2;1 pore mutants. Several, but not all, mutants with a NIP-like selectivity filter promoted yeast growth on urea or ammonia as sole sources of nitrogen. TIP-like mutants conducted urea but not NH(3), and a residue without direct contact to the pore lumen was critical for conduction in the mutants.

Item Type: Article
Erschienen: 2008
Creators: Dynowski, Marek and Mayer, Maria and Moran, Oscar and Ludewig, Uwe
Title: Molecular determinants of ammonia and urea conductance in plant aquaporin homologs.
Language: English
Abstract:

Aquaporins and/or aquaglyceroporins regulate the permeability of plant membranes to water and small, uncharged molecules. Using molecular simulations with a plant plasma membrane aquaporin tetramer, the residues in the channel constriction region were identified as the crucial determinants of ammonia and urea conductance. The impact of these residues was experimentally verified using AtPIP2;1 pore mutants. Several, but not all, mutants with a NIP-like selectivity filter promoted yeast growth on urea or ammonia as sole sources of nitrogen. TIP-like mutants conducted urea but not NH(3), and a residue without direct contact to the pore lumen was critical for conduction in the mutants.

Journal or Publication Title: FEBS letters
Volume: 582
Number: 16
Divisions: 10 Department of Biology > Plant Nutrition and Biomass
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10 Department of Biology
Date Deposited: 16 Mar 2010 13:12
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