Leonhardt, H. ; Page, A. W. ; Weier, H. U. ; Bestor, T. H. (1992)
A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei.
In: Cell, 71 (5)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Tissue-specific patterns of methylated deoxycytidine residues in the mammalian genome are preserved by postreplicative methylation of newly synthesized DNA. DNA methyltransferase (MTase) is here shown to associate with replication foci during S phase but to display a diffuse nucleoplasmic distribution in non-S phase cells. Analysis of DNA MTase-beta-galactosidase fusion proteins has shown that association with replication foci is mediated by a novel targeting sequence located near the N-terminus of DNA MTase. This sequence has the properties expected of a targeting sequence in that it is not required for enzymatic activity, prevents proper targeting when deleted, and, when fused to beta-galactosidase, causes the fusion protein to associate with replication foci in a cell cycle-dependent manner.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 1992 |
| Autor(en): | Leonhardt, H. ; Page, A. W. ; Weier, H. U. ; Bestor, T. H. |
| Art des Eintrags: | Bibliographie |
| Titel: | A targeting sequence directs DNA methyltransferase to sites of DNA replication in mammalian nuclei. |
| Sprache: | Englisch |
| Publikationsjahr: | 1992 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Cell |
| Jahrgang/Volume einer Zeitschrift: | 71 |
| (Heft-)Nummer: | 5 |
| URL / URN: | http://www.cardoso-lab.org/publications/Leonhardt_1992.pdf |
| Kurzbeschreibung (Abstract): | Tissue-specific patterns of methylated deoxycytidine residues in the mammalian genome are preserved by postreplicative methylation of newly synthesized DNA. DNA methyltransferase (MTase) is here shown to associate with replication foci during S phase but to display a diffuse nucleoplasmic distribution in non-S phase cells. Analysis of DNA MTase-beta-galactosidase fusion proteins has shown that association with replication foci is mediated by a novel targeting sequence located near the N-terminus of DNA MTase. This sequence has the properties expected of a targeting sequence in that it is not required for enzymatic activity, prevents proper targeting when deleted, and, when fused to beta-galactosidase, causes the fusion protein to associate with replication foci in a cell cycle-dependent manner. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie ?? fb10_zoologie ?? 10 Fachbereich Biologie > Cell Biology and Epigenetics |
| Hinterlegungsdatum: | 05 Mär 2010 14:54 |
| Letzte Änderung: | 17 Dez 2018 15:19 |
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