TU Darmstadt / ULB / TUbiblio

Reversal of terminal differentiation and control of DNA replication: cyclin A and Cdk2 specifically localize at subnuclear sites of DNA replication.

Cardoso, M. Cristina ; Leonhardt, H. ; Nadal-Ginard, B. (1993)
Reversal of terminal differentiation and control of DNA replication: cyclin A and Cdk2 specifically localize at subnuclear sites of DNA replication.
In: Cell, 74 (6)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

DNA replication in mammalian cells occurs in discrete nuclear foci. Here we show that terminally differentiated myotubes can be induced to reenter S phase and show the same pattern of replication foci as cycling cells. We used this cellular system to analyze the interaction of cell cycle proteins with these foci in vivo. Cyclin A and cdk2, but not cyclin B1 and cdc2, were specifically localized at nuclear replication foci, just like the replication protein proliferating cell nuclear antigen. A potential target of cyclin A and cdk2 is the 34 kd subunit of replication protein A (RPA34). In contrast with the 70 kd subunit, which localizes to the foci, RPA34 was not detected at these replication sites, which may reflect a transient interaction. The specific localization of cyclin A and cdk2 at nuclear replication foci provides a direct link between cell cycle regulation and DNA replication.

Typ des Eintrags: Artikel
Erschienen: 1993
Autor(en): Cardoso, M. Cristina ; Leonhardt, H. ; Nadal-Ginard, B.
Art des Eintrags: Bibliographie
Titel: Reversal of terminal differentiation and control of DNA replication: cyclin A and Cdk2 specifically localize at subnuclear sites of DNA replication.
Sprache: Englisch
Publikationsjahr: 1993
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Cell
Jahrgang/Volume einer Zeitschrift: 74
(Heft-)Nummer: 6
URL / URN: http://www.cardoso-lab.org/publications/Cardoso_1993.pdf
Kurzbeschreibung (Abstract):

DNA replication in mammalian cells occurs in discrete nuclear foci. Here we show that terminally differentiated myotubes can be induced to reenter S phase and show the same pattern of replication foci as cycling cells. We used this cellular system to analyze the interaction of cell cycle proteins with these foci in vivo. Cyclin A and cdk2, but not cyclin B1 and cdc2, were specifically localized at nuclear replication foci, just like the replication protein proliferating cell nuclear antigen. A potential target of cyclin A and cdk2 is the 34 kd subunit of replication protein A (RPA34). In contrast with the 70 kd subunit, which localizes to the foci, RPA34 was not detected at these replication sites, which may reflect a transient interaction. The specific localization of cyclin A and cdk2 at nuclear replication foci provides a direct link between cell cycle regulation and DNA replication.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie > Cell Biology and Epigenetics
?? fb10_zoologie ??
10 Fachbereich Biologie
Hinterlegungsdatum: 05 Mär 2010 15:39
Letzte Änderung: 13 Nov 2014 08:10
PPN:
Export:
Suche nach Titel in: TUfind oder in Google
Frage zum Eintrag Frage zum Eintrag

Optionen (nur für Redakteure)
Redaktionelle Details anzeigen Redaktionelle Details anzeigen