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Structure and function of the mouse DNA methyltransferase gene: Dnmt1 shows a tripartite structure.

Margot, J B. ; Aguirre-Arteta, A M. ; Di Giacco, B V. ; Pradhan, S ; Roberts, R J. ; Cardoso, M Cristina ; Leonhardt, H :
Structure and function of the mouse DNA methyltransferase gene: Dnmt1 shows a tripartite structure.
[Online-Edition: http://www.cardoso-lab.org/publications/Margot_2000.pdf]
In: Journal of molecular biology, 297 (2) pp. 293-300. ISSN 0022-2836
[Artikel], (2000)

Offizielle URL: http://www.cardoso-lab.org/publications/Margot_2000.pdf

Kurzbeschreibung (Abstract)

Dnmt1 is the predominant DNA methyltransferase (MTase) in mammals. The C-terminal domain of Dnmt1 clearly shares sequence similarity with many prokaryotic 5mC methyltransferases, and had been proposed to be sufficient for catalytic activity. We show here by deletion analysis that the C-terminal domain alone is not sufficient for methylating activity, but that a large part of the N-terminal domain is required in addition. Since this complex structure of Dnmt1 raises issues about its evolutionary origin, we have compared several eukaryotic MTases and have determined the genomic organization of the mouse Dnmt1 gene. The 5' most part of the N-terminal domain is dispensible for enzyme activity, includes the major nuclear import signal and comprises tissue-specific exons. Interestingly, the functional subdivision of Dnmt1 correlates well with the structure of the Dnmt1 gene in terms of intron/exon size distribution as well as sequence conservation. Our results, based on functional, structural and sequence comparison data, suggest that the gene has evolved from the fusion of at least three genes.

Typ des Eintrags: Artikel
Erschienen: 2000
Autor(en): Margot, J B. ; Aguirre-Arteta, A M. ; Di Giacco, B V. ; Pradhan, S ; Roberts, R J. ; Cardoso, M Cristina ; Leonhardt, H
Titel: Structure and function of the mouse DNA methyltransferase gene: Dnmt1 shows a tripartite structure.
Sprache: Deutsch
Kurzbeschreibung (Abstract):

Dnmt1 is the predominant DNA methyltransferase (MTase) in mammals. The C-terminal domain of Dnmt1 clearly shares sequence similarity with many prokaryotic 5mC methyltransferases, and had been proposed to be sufficient for catalytic activity. We show here by deletion analysis that the C-terminal domain alone is not sufficient for methylating activity, but that a large part of the N-terminal domain is required in addition. Since this complex structure of Dnmt1 raises issues about its evolutionary origin, we have compared several eukaryotic MTases and have determined the genomic organization of the mouse Dnmt1 gene. The 5' most part of the N-terminal domain is dispensible for enzyme activity, includes the major nuclear import signal and comprises tissue-specific exons. Interestingly, the functional subdivision of Dnmt1 correlates well with the structure of the Dnmt1 gene in terms of intron/exon size distribution as well as sequence conservation. Our results, based on functional, structural and sequence comparison data, suggest that the gene has evolved from the fusion of at least three genes.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Journal of molecular biology
Band: 297
(Heft-)Nummer: 2
Fachbereich(e)/-gebiet(e): Fachbereich Biologie, Biology > Zellbiologie und Epigenetik, Cell Biology and Epigenetics
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Fachbereich Biologie, Biology
Hinterlegungsdatum: 05 Mär 2010 15:57
Offizielle URL: http://www.cardoso-lab.org/publications/Margot_2000.pdf
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