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Expression of an alternative Dnmt1 isoform during muscle differentiation.

Aguirre-Arteta, A M. ; Grunewald, I ; Cardoso, M Cristina ; Leonhardt, H :
Expression of an alternative Dnmt1 isoform during muscle differentiation.
[Online-Edition: http://www.cardoso-lab.org/publications/Aguirre-Arteta_2000....]
In: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, 11 (10) pp. 551-9. ISSN 1044-9523
[Artikel], (2000)

Offizielle URL: http://www.cardoso-lab.org/publications/Aguirre-Arteta_2000....

Kurzbeschreibung (Abstract)

The methylation pattern of genomic DNA undergoes dramatic changes during mammalian development, with extensive de novo methylation occurring during gametogenesis and after implantation. We identified an alternative Dnmt1 transcript in skeletal muscle by Northern blot analysis and cloned the corresponding cDNA by rapid amplification of cDNA ends and reverse transcription-PCR. Using an in vitro skeletal muscle differentiation system, we show that this alternative Dnmt1 isoform is specifically expressed in differentiated myotubes, whereas the ubiquitously expressed isoform is down-regulated during myogenesis. Sequence analysis showed that this skeletal Dnmt1 isoform is identical to the one present in testis, which had been described as untranslatable. Here we present evidence that this alternative Dnmt1 transcript present in testis and skeletal muscle is translated despite the presence of several out-of-frame upstream ATGs and gives rise to a shorter Dnmt1 isoform, which could play an active role in the change of DNA methylation patterns during gametogenesis and myogenesis.

Typ des Eintrags: Artikel
Erschienen: 2000
Autor(en): Aguirre-Arteta, A M. ; Grunewald, I ; Cardoso, M Cristina ; Leonhardt, H
Titel: Expression of an alternative Dnmt1 isoform during muscle differentiation.
Sprache: Englisch
Kurzbeschreibung (Abstract):

The methylation pattern of genomic DNA undergoes dramatic changes during mammalian development, with extensive de novo methylation occurring during gametogenesis and after implantation. We identified an alternative Dnmt1 transcript in skeletal muscle by Northern blot analysis and cloned the corresponding cDNA by rapid amplification of cDNA ends and reverse transcription-PCR. Using an in vitro skeletal muscle differentiation system, we show that this alternative Dnmt1 isoform is specifically expressed in differentiated myotubes, whereas the ubiquitously expressed isoform is down-regulated during myogenesis. Sequence analysis showed that this skeletal Dnmt1 isoform is identical to the one present in testis, which had been described as untranslatable. Here we present evidence that this alternative Dnmt1 transcript present in testis and skeletal muscle is translated despite the presence of several out-of-frame upstream ATGs and gives rise to a shorter Dnmt1 isoform, which could play an active role in the change of DNA methylation patterns during gametogenesis and myogenesis.

Titel der Zeitschrift, Zeitung oder Schriftenreihe: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
Band: 11
(Heft-)Nummer: 10
Fachbereich(e)/-gebiet(e): Fachbereich Biologie, Biology > Zellbiologie und Epigenetik, Cell Biology and Epigenetics
Fachbereich Biologie, Biology
Hinterlegungsdatum: 05 Mär 2010 16:01
Offizielle URL: http://www.cardoso-lab.org/publications/Aguirre-Arteta_2000....
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