TU Darmstadt / ULB / TUbiblio

Replication-independent chromatin loading of Dnmt1 during G2 and M phases.

Easwaran, Hariharan P. and Schermelleh, Lothar and Leonhardt, Heinrich and Cardoso, M Cristina (2004):
Replication-independent chromatin loading of Dnmt1 during G2 and M phases.
5, In: EMBO reports, (12), pp. 1181-6, ISSN 1469-221X, [Online-Edition: http://www.cardoso-lab.org/publications/Easwaran_2004.pdf],
[Article]

Abstract

The major DNA methyltransferase, Dnmt1, associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand. In view of the slow kinetics of Dnmt1 in vitro versus the fast progression of the replication fork, we have tested whether Dnmt1 associates with chromatin beyond S phase. Using time-lapse microscopy of mammalian cells expressing green-fluorescent-protein-tagged Dnmt1 and DsRed-tagged DNA Ligase I as a cell cycle progression marker, we have found that Dnmt1 associates with chromatin during G2 and M. This association is mediated by a specific targeting sequence, shows strong preference for constitutive but not facultative heterochromatin and is independent of heterochromatin-specific histone H3 Lys 9 trimethylation, SUV39H and HP1. Moreover, photobleaching analyses showed that Dnmt1 is continuously loaded onto chromatin throughout G2 and M, indicating a replication-independent role of Dnmt1 that could represent a novel and separate pathway to maintain DNA methylation.

Item Type: Article
Erschienen: 2004
Creators: Easwaran, Hariharan P. and Schermelleh, Lothar and Leonhardt, Heinrich and Cardoso, M Cristina
Title: Replication-independent chromatin loading of Dnmt1 during G2 and M phases.
Language: German
Abstract:

The major DNA methyltransferase, Dnmt1, associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand. In view of the slow kinetics of Dnmt1 in vitro versus the fast progression of the replication fork, we have tested whether Dnmt1 associates with chromatin beyond S phase. Using time-lapse microscopy of mammalian cells expressing green-fluorescent-protein-tagged Dnmt1 and DsRed-tagged DNA Ligase I as a cell cycle progression marker, we have found that Dnmt1 associates with chromatin during G2 and M. This association is mediated by a specific targeting sequence, shows strong preference for constitutive but not facultative heterochromatin and is independent of heterochromatin-specific histone H3 Lys 9 trimethylation, SUV39H and HP1. Moreover, photobleaching analyses showed that Dnmt1 is continuously loaded onto chromatin throughout G2 and M, indicating a replication-independent role of Dnmt1 that could represent a novel and separate pathway to maintain DNA methylation.

Journal or Publication Title: EMBO reports
Volume: 5
Number: 12
Divisions: 10 Department of Biology > Cell Biology and Epigenetics
?? fb10_zoologie ??
10 Department of Biology
Date Deposited: 06 Mar 2010 07:39
Official URL: http://www.cardoso-lab.org/publications/Easwaran_2004.pdf
Export:
Suche nach Titel in: TUfind oder in Google
Send an inquiry Send an inquiry

Options (only for editors)

View Item View Item