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Histone acetylation increases chromatin accessibility.

Görisch, Sabine M. and Wachsmuth, Malte and Tóth, Katalin Fejes and Lichter, Peter and Rippe, Karsten :
Histone acetylation increases chromatin accessibility.
[Online-Edition: http://www.cardoso-lab.org/publications/Goerisch_2005b.pdf]
In: Journal of cell science, 118 (Pt 24) pp. 5825-34. ISSN 0021-9533
[Article] , (2005)

Official URL: http://www.cardoso-lab.org/publications/Goerisch_2005b.pdf

Abstract

In eukaryotes, the interaction of DNA with proteins and supramolecular complexes involved in gene expression is controlled by the dynamic organization of chromatin inasmuch as it defines the DNA accessibility. Here, the nuclear distribution of microinjected fluorescein-labeled dextrans of 42 kDa to 2.5 MDa molecular mass was used to characterize the chromatin accessibility in dependence on histone acetylation. Measurements of the fluorescein-dextran sizes were combined with an image correlation spectroscopy analysis, and three different interphase chromatin condensation states with apparent pore sizes of 16-20 nm, 36-56 nm and 60-100 nm were identified. A reversible change of the chromatin conformation to a uniform 60-100 nm pore size distribution was observed upon increased histone acetylation. This result identifies histone acetylation as a central factor in the dynamic regulation of chromatin accessibility during interphase. In mitotic chromosomes, the chromatin exclusion limit was 10-20 nm and independent of the histone acetylation state.

Item Type: Article
Erschienen: 2005
Creators: Görisch, Sabine M. and Wachsmuth, Malte and Tóth, Katalin Fejes and Lichter, Peter and Rippe, Karsten
Title: Histone acetylation increases chromatin accessibility.
Language: German
Abstract:

In eukaryotes, the interaction of DNA with proteins and supramolecular complexes involved in gene expression is controlled by the dynamic organization of chromatin inasmuch as it defines the DNA accessibility. Here, the nuclear distribution of microinjected fluorescein-labeled dextrans of 42 kDa to 2.5 MDa molecular mass was used to characterize the chromatin accessibility in dependence on histone acetylation. Measurements of the fluorescein-dextran sizes were combined with an image correlation spectroscopy analysis, and three different interphase chromatin condensation states with apparent pore sizes of 16-20 nm, 36-56 nm and 60-100 nm were identified. A reversible change of the chromatin conformation to a uniform 60-100 nm pore size distribution was observed upon increased histone acetylation. This result identifies histone acetylation as a central factor in the dynamic regulation of chromatin accessibility during interphase. In mitotic chromosomes, the chromatin exclusion limit was 10-20 nm and independent of the histone acetylation state.

Journal or Publication Title: Journal of cell science
Volume: 118
Number: Pt 24
Divisions: 10 Department of Biology > Cell Biology and Epigenetics
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10 Department of Biology
Date Deposited: 06 Mar 2010 07:53
Official URL: http://www.cardoso-lab.org/publications/Goerisch_2005b.pdf
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