Görisch, Sabine M. ; Wachsmuth, Malte ; Tóth, Katalin Fejes ; Lichter, Peter ; Rippe, Karsten (2005)
Histone acetylation increases chromatin accessibility.
In: Journal of cell science, 118 (Pt 24)
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
In eukaryotes, the interaction of DNA with proteins and supramolecular complexes involved in gene expression is controlled by the dynamic organization of chromatin inasmuch as it defines the DNA accessibility. Here, the nuclear distribution of microinjected fluorescein-labeled dextrans of 42 kDa to 2.5 MDa molecular mass was used to characterize the chromatin accessibility in dependence on histone acetylation. Measurements of the fluorescein-dextran sizes were combined with an image correlation spectroscopy analysis, and three different interphase chromatin condensation states with apparent pore sizes of 16-20 nm, 36-56 nm and 60-100 nm were identified. A reversible change of the chromatin conformation to a uniform 60-100 nm pore size distribution was observed upon increased histone acetylation. This result identifies histone acetylation as a central factor in the dynamic regulation of chromatin accessibility during interphase. In mitotic chromosomes, the chromatin exclusion limit was 10-20 nm and independent of the histone acetylation state.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2005 |
| Autor(en): | Görisch, Sabine M. ; Wachsmuth, Malte ; Tóth, Katalin Fejes ; Lichter, Peter ; Rippe, Karsten |
| Art des Eintrags: | Bibliographie |
| Titel: | Histone acetylation increases chromatin accessibility. |
| Sprache: | Deutsch |
| Publikationsjahr: | 2005 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Journal of cell science |
| Jahrgang/Volume einer Zeitschrift: | 118 |
| (Heft-)Nummer: | Pt 24 |
| URL / URN: | http://www.cardoso-lab.org/publications/Goerisch_2005b.pdf |
| Kurzbeschreibung (Abstract): | In eukaryotes, the interaction of DNA with proteins and supramolecular complexes involved in gene expression is controlled by the dynamic organization of chromatin inasmuch as it defines the DNA accessibility. Here, the nuclear distribution of microinjected fluorescein-labeled dextrans of 42 kDa to 2.5 MDa molecular mass was used to characterize the chromatin accessibility in dependence on histone acetylation. Measurements of the fluorescein-dextran sizes were combined with an image correlation spectroscopy analysis, and three different interphase chromatin condensation states with apparent pore sizes of 16-20 nm, 36-56 nm and 60-100 nm were identified. A reversible change of the chromatin conformation to a uniform 60-100 nm pore size distribution was observed upon increased histone acetylation. This result identifies histone acetylation as a central factor in the dynamic regulation of chromatin accessibility during interphase. In mitotic chromosomes, the chromatin exclusion limit was 10-20 nm and independent of the histone acetylation state. |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie > Cell Biology and Epigenetics ?? fb10_zoologie ?? 10 Fachbereich Biologie |
| Hinterlegungsdatum: | 06 Mär 2010 07:53 |
| Letzte Änderung: | 05 Mär 2013 09:32 |
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