Rothbauer, Ulrich and Zolghadr, Kourosh and Tillib, Sergei and Nowak, Danny and Schermelleh, Lothar and Gahl, Anja and Backmann, Natalija and Conrath, Katja and Muyldermans, Serge and Cardoso, M Cristina and Leonhardt, Heinrich (2006):
Targeting and tracing antigens in live cells with fluorescent nanobodies.
In: Nature methods, pp. 887-9, 3, (11), ISSN 1548-7091, [Online-Edition: http://www.cardoso-lab.org/publications/Rothbauer%20et%20al....],
[Article]
Abstract
We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.
| Item Type: | Article |
|---|---|
| Erschienen: | 2006 |
| Creators: | Rothbauer, Ulrich and Zolghadr, Kourosh and Tillib, Sergei and Nowak, Danny and Schermelleh, Lothar and Gahl, Anja and Backmann, Natalija and Conrath, Katja and Muyldermans, Serge and Cardoso, M Cristina and Leonhardt, Heinrich |
| Title: | Targeting and tracing antigens in live cells with fluorescent nanobodies. |
| Language: | German |
| Abstract: | We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion. |
| Journal or Publication Title: | Nature methods |
| Volume: | 3 |
| Number: | 11 |
| Divisions: | 10 Department of Biology > Cell Biology and Epigenetics ?? fb10_zoologie ?? 10 Department of Biology |
| Date Deposited: | 06 Mar 2010 08:16 |
| Official URL: | http://www.cardoso-lab.org/publications/Rothbauer%20et%20al.... |
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