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Targeting and tracing antigens in live cells with fluorescent nanobodies.

Rothbauer, Ulrich and Zolghadr, Kourosh and Tillib, Sergei and Nowak, Danny and Schermelleh, Lothar and Gahl, Anja and Backmann, Natalija and Conrath, Katja and Muyldermans, Serge and Cardoso, M Cristina and Leonhardt, Heinrich (2006):
Targeting and tracing antigens in live cells with fluorescent nanobodies.
In: Nature methods, pp. 887-9, 3, (11), ISSN 1548-7091, [Online-Edition: http://www.cardoso-lab.org/publications/Rothbauer%20et%20al....],
[Article]

Abstract

We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.

Item Type: Article
Erschienen: 2006
Creators: Rothbauer, Ulrich and Zolghadr, Kourosh and Tillib, Sergei and Nowak, Danny and Schermelleh, Lothar and Gahl, Anja and Backmann, Natalija and Conrath, Katja and Muyldermans, Serge and Cardoso, M Cristina and Leonhardt, Heinrich
Title: Targeting and tracing antigens in live cells with fluorescent nanobodies.
Language: German
Abstract:

We fused the epitope-recognizing fragment of heavy-chain antibodies from Camelidae sp. with fluorescent proteins to generate fluorescent, antigen-binding nanobodies (chromobodies) that can be expressed in living cells. We demonstrate that chromobodies can recognize and trace antigens in different subcellular compartments throughout S phase and mitosis. Chromobodies should enable new functional studies, as potentially any antigenic structure can be targeted and traced in living cells in this fashion.

Journal or Publication Title: Nature methods
Volume: 3
Number: 11
Divisions: 10 Department of Biology > Cell Biology and Epigenetics
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10 Department of Biology
Date Deposited: 06 Mar 2010 08:16
Official URL: http://www.cardoso-lab.org/publications/Rothbauer%20et%20al....
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