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Modulation of muscle contraction by a cell-permeable peptide.

Tünnemann, Gisela and Karczewski, Peter and Haase, Hannelore and Cardoso, M Cristina and Morano, Ingo (2007):
Modulation of muscle contraction by a cell-permeable peptide.
85, In: Journal of molecular medicine, (12), pp. 1405-12, ISSN 0946-2716, [Online-Edition: http://www.cardoso-lab.org/publications/Tunnemann%202007-EPu...],
[Article]

Abstract

In contrast to immortal cell lines, primary cells are hardly susceptible to intracellular delivery methods such as transfection. In this study, we evaluated the direct delivery of several cell-permeable peptides under noninvasive conditions into living primary adult rat cardiomyocytes. We specifically monitored the functional effects of a cell-permeable peptide containing the 15 amino acid N-terminal peptide from human ventricular light chain-1 (VLC-1) on contraction and intracellular Ca2+ signals after electrical stimulation in primary adult cardiomyocytes. The transducible VLC-1 variant was taken up by cardiomyocytes within 5 min with more than 95% efficiency and localized to sarcomeric structures. Analysis of the functional effects of the cell-permeable VLC-1 revealed an enhancement of the intrinsic contractility of cardiomyocytes without affecting the intracellular Ca2+. Therefore, peptide transduction mediated by cell-penetrating peptides represents not only a unique strategy to enhance heart muscle function with no secondary effect on intracellular Ca2+ but also an invaluable tool for the modulation and manipulation of protein interactions in general and in primary cells.

Item Type: Article
Erschienen: 2007
Creators: Tünnemann, Gisela and Karczewski, Peter and Haase, Hannelore and Cardoso, M Cristina and Morano, Ingo
Title: Modulation of muscle contraction by a cell-permeable peptide.
Language: English
Abstract:

In contrast to immortal cell lines, primary cells are hardly susceptible to intracellular delivery methods such as transfection. In this study, we evaluated the direct delivery of several cell-permeable peptides under noninvasive conditions into living primary adult rat cardiomyocytes. We specifically monitored the functional effects of a cell-permeable peptide containing the 15 amino acid N-terminal peptide from human ventricular light chain-1 (VLC-1) on contraction and intracellular Ca2+ signals after electrical stimulation in primary adult cardiomyocytes. The transducible VLC-1 variant was taken up by cardiomyocytes within 5 min with more than 95% efficiency and localized to sarcomeric structures. Analysis of the functional effects of the cell-permeable VLC-1 revealed an enhancement of the intrinsic contractility of cardiomyocytes without affecting the intracellular Ca2+. Therefore, peptide transduction mediated by cell-penetrating peptides represents not only a unique strategy to enhance heart muscle function with no secondary effect on intracellular Ca2+ but also an invaluable tool for the modulation and manipulation of protein interactions in general and in primary cells.

Journal or Publication Title: Journal of molecular medicine
Volume: 85
Number: 12
Divisions: 10 Department of Biology > Cell Biology and Epigenetics
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10 Department of Biology
Date Deposited: 06 Mar 2010 15:45
Official URL: http://www.cardoso-lab.org/publications/Tunnemann%202007-EPu...
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