Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy.

Schermelleh, Lothar and Carlton, Peter M. and Haase, Sebastian and Shao, Lin and Winoto, Lukman and Kner, Peter and Burke, Brian and Cardoso, M Cristina and Agard, David A and Gustafsson, Mats G. L. and Leonhardt, Heinrich and Sedat, John W. :
Subdiffraction multicolor imaging of the nuclear periphery with 3D structured illumination microscopy.
[Online-Edition: http://www.cardoso-lab.org/publications/Schermelleh_2008.pdf]
In: Science, 320 (5881) pp. 1332-6. ISSN 1095-9203
[Article], (2008)

Abstract

Fluorescence light microscopy allows multicolor visualization of cellular components with high specificity, but its utility has until recently been constrained by the intrinsic limit of spatial resolution. We applied three-dimensional structured illumination microscopy (3D-SIM) to circumvent this limit and to study the mammalian nucleus. By simultaneously imaging chromatin, nuclear lamina, and the nuclear pore complex (NPC), we observed several features that escape detection by conventional microscopy. We could resolve single NPCs that colocalized with channels in the lamin network and peripheral heterochromatin. We could differentially localize distinct NPC components and detect double-layered invaginations of the nuclear envelope in prophase as previously seen only by electron microscopy. Multicolor 3D-SIM opens new and facile possibilities to analyze subcellular structures beyond the diffraction limit of the emitted light.

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