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Equine arteritis virus is delivered to an acidic compartment of host cells via clathrin-dependent endocytosis.

Nitschke, Matthias and Korte, Thomas and Tielesch, Claudia and Ter-Avetisyan, Gohar and Tünnemann, Gisela and Cardoso, M Cristina and Veit, Michael and Herrmann, Andreas (2008):
Equine arteritis virus is delivered to an acidic compartment of host cells via clathrin-dependent endocytosis.
In: Virology, pp. 248-54, 377, (2), ISSN 0042-6822, [Online-Edition: http://www.cardoso-lab.org/publications/Nitschke_2008.pdf],
[Article]

Abstract

Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae. Infection by EAV requires the release of the viral genome by fusion with the respective target membrane of the host cell. We have investigated the entry pathway of EAV into Baby Hamster Kidney cells (BHK). Infection of cells assessed by the plaque reduction assay was strongly inhibited by substances which interfere with clathrin-dependent endocytosis and by lysosomotropic compounds. Furthermore, infection of BHK cells was suppressed when clathrin-dependent endocytosis was inhibited by expression of antisense RNA of the clathrin-heavy chain before infection. These results strongly suggest that EAV is taken up via clathrin-dependent endocytosis and is delivered to acidic endosomal compartments.

Item Type: Article
Erschienen: 2008
Creators: Nitschke, Matthias and Korte, Thomas and Tielesch, Claudia and Ter-Avetisyan, Gohar and Tünnemann, Gisela and Cardoso, M Cristina and Veit, Michael and Herrmann, Andreas
Title: Equine arteritis virus is delivered to an acidic compartment of host cells via clathrin-dependent endocytosis.
Language: English
Abstract:

Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae. Infection by EAV requires the release of the viral genome by fusion with the respective target membrane of the host cell. We have investigated the entry pathway of EAV into Baby Hamster Kidney cells (BHK). Infection of cells assessed by the plaque reduction assay was strongly inhibited by substances which interfere with clathrin-dependent endocytosis and by lysosomotropic compounds. Furthermore, infection of BHK cells was suppressed when clathrin-dependent endocytosis was inhibited by expression of antisense RNA of the clathrin-heavy chain before infection. These results strongly suggest that EAV is taken up via clathrin-dependent endocytosis and is delivered to acidic endosomal compartments.

Journal or Publication Title: Virology
Volume: 377
Number: 2
Divisions: 10 Department of Biology > Cell Biology and Epigenetics
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10 Department of Biology
Date Deposited: 06 Mar 2010 15:59
Official URL: http://www.cardoso-lab.org/publications/Nitschke_2008.pdf
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