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Knock-down of GFR-alpha-4 expression by RNA interference affects the development of retinal cell types in three-dimensional histotypic retinal spheres

Rothermel, Andree ; Burghardt, Mirjam ; Volpert, Katja ; Robitzki, Andrea ; Layer, Paul G. ; Lantzsch, Christina (2006)
Knock-down of GFR-alpha-4 expression by RNA interference affects the development of retinal cell types in three-dimensional histotypic retinal spheres.
In: Investigative ophthalmology & visual science, 47 (6)
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

Abstract PURPOSE:

To determine the role of glial cell line-derived neurotropic factor family receptor alpha 4 (GFRalpha4) during retinogenesis in a three-dimensional histiotypic in vitro model of the embryonic chicken retina. METHODS:

Retinal spheres were cultured from dissociated 6-day-old chicken retina under permanent rotation and transfected with GFRalpha4 siRNA at culture day 2. Alterations on proliferation, apoptosis, and differentiation were determined by semiquantitative RT-PCR, in situ hybridization, and immunohistochemistry after 24, 48, and 72 hours. RESULTS:

In contrast to control cultures, retinal spheres transfected with GFRalpha4 siRNA showed reduced GFRalpha4 mRNA expression of only 38% after 24 hours, 3% after 48 hours, and 5% after 72 hours. Based on the suppression of GFRalpha4, a decline in proliferating cells from 10% to 4.8% even after 24 hours and a reduction of sphere size by up to 25% at later culture stages were observed. Moreover, the number of Pax 6-positive amacrine, ganglion, and horizontal cells was significantly decreased from 36% to 16% in GFRalpha4 siRNA-transfected retinal spheres 72 hours after transfection. Additionally, GFRalpha4 gene silencing affected the development of different types of photoreceptors, as revealed by a significant decrease of blue opsin mRNA expression from 29% to 2%, whereas green opsin mRNA and the number rho4D2-positive photoreceptors were significantly increased. CONCLUSIONS:

These data showed for the first time that GFRalpha4 plays an essential role in regulating, at least in vitro, the development and differentiation of various cell types during retinogenesis.

Typ des Eintrags: Artikel
Erschienen: 2006
Autor(en): Rothermel, Andree ; Burghardt, Mirjam ; Volpert, Katja ; Robitzki, Andrea ; Layer, Paul G. ; Lantzsch, Christina
Art des Eintrags: Bibliographie
Titel: Knock-down of GFR-alpha-4 expression by RNA interference affects the development of retinal cell types in three-dimensional histotypic retinal spheres
Sprache: Englisch
Publikationsjahr: 2006
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Investigative ophthalmology & visual science
Jahrgang/Volume einer Zeitschrift: 47
(Heft-)Nummer: 6
Kurzbeschreibung (Abstract):

Abstract PURPOSE:

To determine the role of glial cell line-derived neurotropic factor family receptor alpha 4 (GFRalpha4) during retinogenesis in a three-dimensional histiotypic in vitro model of the embryonic chicken retina. METHODS:

Retinal spheres were cultured from dissociated 6-day-old chicken retina under permanent rotation and transfected with GFRalpha4 siRNA at culture day 2. Alterations on proliferation, apoptosis, and differentiation were determined by semiquantitative RT-PCR, in situ hybridization, and immunohistochemistry after 24, 48, and 72 hours. RESULTS:

In contrast to control cultures, retinal spheres transfected with GFRalpha4 siRNA showed reduced GFRalpha4 mRNA expression of only 38% after 24 hours, 3% after 48 hours, and 5% after 72 hours. Based on the suppression of GFRalpha4, a decline in proliferating cells from 10% to 4.8% even after 24 hours and a reduction of sphere size by up to 25% at later culture stages were observed. Moreover, the number of Pax 6-positive amacrine, ganglion, and horizontal cells was significantly decreased from 36% to 16% in GFRalpha4 siRNA-transfected retinal spheres 72 hours after transfection. Additionally, GFRalpha4 gene silencing affected the development of different types of photoreceptors, as revealed by a significant decrease of blue opsin mRNA expression from 29% to 2%, whereas green opsin mRNA and the number rho4D2-positive photoreceptors were significantly increased. CONCLUSIONS:

These data showed for the first time that GFRalpha4 plays an essential role in regulating, at least in vitro, the development and differentiation of various cell types during retinogenesis.

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
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10 Fachbereich Biologie > Developmental Biology and Neurogenetics
Hinterlegungsdatum: 20 Nov 2008 08:21
Letzte Änderung: 20 Feb 2020 13:24
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