Gwozdz, Paul V. ; Harberts, Jann ; Zierold, Robert ; Blick, Robert H. (2023)
Label-free single-cell counting and characterization in the GHz-range.
In: Frequenz, 2022, 76 (11-12)
doi: 10.26083/tuprints-00023216
Artikel, Zweitveröffentlichung, Verlagsversion
Kurzbeschreibung (Abstract)
We demonstrate operation of a micropore based flow cytometer in the radio-frequency range. Apart from simply counting micron sized particles, such as cells, with close to nano-second resolution this counter offers the additional benefit of delivering insight into the intracellular environment. Such non-invasive screening of the cell’s interior based on analysing amplitude and phase of the signal is helpful in characterizing the biological activity of cells. In detail we are using heterodyne mixing to demodulate the temporal impedance changes, which are induced by cells translocating through a micropore embedded in a radio-frequency circuit. This allows us to measure every amplitude and phase modulation induced by a translocation event. Herein, we compare the Jurkat cells (human T lymphocytes) recordings with a control group of polystyrene beads. As the cells are measured on a single cell level, the variations on the measured amplitude and phase signals are used, herein, to sense morphological cell changes in real time.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2023 |
| Autor(en): | Gwozdz, Paul V. ; Harberts, Jann ; Zierold, Robert ; Blick, Robert H. |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Label-free single-cell counting and characterization in the GHz-range |
| Sprache: | Englisch |
| Publikationsjahr: | 2023 |
| Ort: | Darmstadt |
| Publikationsdatum der Erstveröffentlichung: | 2022 |
| Verlag: | De Gruyter |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Frequenz |
| Jahrgang/Volume einer Zeitschrift: | 76 |
| (Heft-)Nummer: | 11-12 |
| DOI: | 10.26083/tuprints-00023216 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/23216 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichung |
| Kurzbeschreibung (Abstract): | We demonstrate operation of a micropore based flow cytometer in the radio-frequency range. Apart from simply counting micron sized particles, such as cells, with close to nano-second resolution this counter offers the additional benefit of delivering insight into the intracellular environment. Such non-invasive screening of the cell’s interior based on analysing amplitude and phase of the signal is helpful in characterizing the biological activity of cells. In detail we are using heterodyne mixing to demodulate the temporal impedance changes, which are induced by cells translocating through a micropore embedded in a radio-frequency circuit. This allows us to measure every amplitude and phase modulation induced by a translocation event. Herein, we compare the Jurkat cells (human T lymphocytes) recordings with a control group of polystyrene beads. As the cells are measured on a single cell level, the variations on the measured amplitude and phase signals are used, herein, to sense morphological cell changes in real time. |
| Freie Schlagworte: | radio frequency circuits, single cell counting, spectroscopic probing |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-232167 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 530 Physik 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften und Maschinenbau |
| Fachbereich(e)/-gebiet(e): | 18 Fachbereich Elektrotechnik und Informationstechnik 18 Fachbereich Elektrotechnik und Informationstechnik > Institut für Mikrowellentechnik und Photonik (IMP) |
| Hinterlegungsdatum: | 01 Mär 2023 10:00 |
| Letzte Änderung: | 06 Mär 2023 11:06 |
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