Radstake, Wilhelmina E. ; Gautam, Kiran ; Miranda, Silvana ; Vermeesen, Randy ; Tabury, Kevin ; Rehnberg, Emil ; Buset, Jasmine ; Janssen, Ann ; Leysen, Liselotte ; Neefs, Mieke ; Verslegers, Mieke ; Claesen, Jürgen ; Goethem, Marc-Jan van ; Weber, Uli ; Fournier, Claudia ; Parisi, Alessio ; Brandenburg, Sytze ; Durante, Marco ; Baselet, Bjorn ; Baatout, Sarah (2023)
The Effects of Combined Exposure to Simulated Microgravity, Ionizing Radiation, and Cortisol on the In Vitro Wound Healing Process.
In: Cells, 2023, 12 (2)
doi: 10.26083/tuprints-00023171
Artikel, Zweitveröffentlichung, Verlagsversion
Kurzbeschreibung (Abstract)
Human spaceflight is associated with several health-related issues as a result of long-term exposure to microgravity, ionizing radiation, and higher levels of psychological stress. Frequent reported skin problems in space include rashes, itches, and a delayed wound healing. Access to space is restricted by financial and logistical issues; as a consequence, experimental sample sizes are often small, which limits the generalization of the results. Earth-based simulation models can be used to investigate cellular responses as a result of exposure to certain spaceflight stressors. Here, we describe the development of an in vitro model of the simulated spaceflight environment, which we used to investigate the combined effect of simulated microgravity using the random positioning machine (RPM), ionizing radiation, and stress hormones on the wound-healing capacity of human dermal fibroblasts. Fibroblasts were exposed to cortisol, after which they were irradiated with different radiation qualities (including X-rays, protons, carbon ions, and iron ions) followed by exposure to simulated microgravity using a random positioning machine (RPM). Data related to the inflammatory, proliferation, and remodeling phase of wound healing has been collected. Results show that spaceflight stressors can interfere with the wound healing process at any phase. Moreover, several interactions between the different spaceflight stressors were found. This highlights the complexity that needs to be taken into account when studying the effect of spaceflight stressors on certain biological processes and for the aim of countermeasures development.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2023 |
| Autor(en): | Radstake, Wilhelmina E. ; Gautam, Kiran ; Miranda, Silvana ; Vermeesen, Randy ; Tabury, Kevin ; Rehnberg, Emil ; Buset, Jasmine ; Janssen, Ann ; Leysen, Liselotte ; Neefs, Mieke ; Verslegers, Mieke ; Claesen, Jürgen ; Goethem, Marc-Jan van ; Weber, Uli ; Fournier, Claudia ; Parisi, Alessio ; Brandenburg, Sytze ; Durante, Marco ; Baselet, Bjorn ; Baatout, Sarah |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | The Effects of Combined Exposure to Simulated Microgravity, Ionizing Radiation, and Cortisol on the In Vitro Wound Healing Process |
| Sprache: | Englisch |
| Publikationsjahr: | 2023 |
| Ort: | Darmstadt |
| Publikationsdatum der Erstveröffentlichung: | 2023 |
| Verlag: | MDPI |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Cells |
| Jahrgang/Volume einer Zeitschrift: | 12 |
| (Heft-)Nummer: | 2 |
| Kollation: | 32 Seiten |
| DOI: | 10.26083/tuprints-00023171 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/23171 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichung DeepGreen |
| Kurzbeschreibung (Abstract): | Human spaceflight is associated with several health-related issues as a result of long-term exposure to microgravity, ionizing radiation, and higher levels of psychological stress. Frequent reported skin problems in space include rashes, itches, and a delayed wound healing. Access to space is restricted by financial and logistical issues; as a consequence, experimental sample sizes are often small, which limits the generalization of the results. Earth-based simulation models can be used to investigate cellular responses as a result of exposure to certain spaceflight stressors. Here, we describe the development of an in vitro model of the simulated spaceflight environment, which we used to investigate the combined effect of simulated microgravity using the random positioning machine (RPM), ionizing radiation, and stress hormones on the wound-healing capacity of human dermal fibroblasts. Fibroblasts were exposed to cortisol, after which they were irradiated with different radiation qualities (including X-rays, protons, carbon ions, and iron ions) followed by exposure to simulated microgravity using a random positioning machine (RPM). Data related to the inflammatory, proliferation, and remodeling phase of wound healing has been collected. Results show that spaceflight stressors can interfere with the wound healing process at any phase. Moreover, several interactions between the different spaceflight stressors were found. This highlights the complexity that needs to be taken into account when studying the effect of spaceflight stressors on certain biological processes and for the aim of countermeasures development. |
| Freie Schlagworte: | simulated microgravity, ionizing radiation, iron ions, carbon ions, protons, cortisol, fibroblast, in vitro, wound healing |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-231719 |
| Zusätzliche Informationen: | This article belongs to the Special Issue New insights into Microgravity and Space Biology |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 530 Physik 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin, Gesundheit |
| Fachbereich(e)/-gebiet(e): | 05 Fachbereich Physik 05 Fachbereich Physik > Institut für Physik Kondensierter Materie (IPKM) |
| Hinterlegungsdatum: | 06 Feb 2023 13:23 |
| Letzte Änderung: | 15 Feb 2023 13:27 |
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