Gawlitza, Kornelia ; Wu, Changzhu ; Georgieva, Radostina ; Ansorge-Schumacher, Marion ; Klitzing, Regine von (2022)
Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles.
In: Zeitschrift für Physikalische Chemie, 226 (7-8)
doi: 10.26083/tuprints-00019949
Artikel, Zweitveröffentlichung, Verlagsversion
Kurzbeschreibung (Abstract)
The immobilization of lipase B from Candida antarctica(CalB) within micronsized poly-N-Isopropylacrylamide (p-NIPAM) microgel particles with a crosslinker content of 5% is reported. The immobilization of the enzyme was reached by an exchange from polar to organic solvents. After determining the embedded amount of CalB within the polymer network, an enhanced specificactivity inn-hexane was obtained. Due to the thermoresponsibility of the polymer particles, the activity reaction was done at 25°C and 50°C. The results presented show that the reversiblecollapse of the microgel leads to a decreased activity with increasing temperature. Hence, p-NIPAMmicrogels display a good opportunity to tailor the activity of CalB. An interesting side effect is that CalB presents a suitable probe to estimate the mesh size of the polymer network, since itpenetrates in the unlabeled form but not after labeling with FITC.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2022 |
| Autor(en): | Gawlitza, Kornelia ; Wu, Changzhu ; Georgieva, Radostina ; Ansorge-Schumacher, Marion ; Klitzing, Regine von |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Temperature Controlled Activity of Lipase B from Candida Antarctica after Immobilization within p-NIPAM Microgel Particles |
| Sprache: | Englisch |
| Publikationsjahr: | 2022 |
| Ort: | Darmstadt |
| Verlag: | De Gruyter |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Zeitschrift für Physikalische Chemie |
| Jahrgang/Volume einer Zeitschrift: | 226 |
| (Heft-)Nummer: | 7-8 |
| DOI: | 10.26083/tuprints-00019949 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/19949 |
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| Herkunft: | Zweitveröffentlichungsservice |
| Kurzbeschreibung (Abstract): | The immobilization of lipase B from Candida antarctica(CalB) within micronsized poly-N-Isopropylacrylamide (p-NIPAM) microgel particles with a crosslinker content of 5% is reported. The immobilization of the enzyme was reached by an exchange from polar to organic solvents. After determining the embedded amount of CalB within the polymer network, an enhanced specificactivity inn-hexane was obtained. Due to the thermoresponsibility of the polymer particles, the activity reaction was done at 25°C and 50°C. The results presented show that the reversiblecollapse of the microgel leads to a decreased activity with increasing temperature. Hence, p-NIPAMmicrogels display a good opportunity to tailor the activity of CalB. An interesting side effect is that CalB presents a suitable probe to estimate the mesh size of the polymer network, since itpenetrates in the unlabeled form but not after labeling with FITC. |
| Freie Schlagworte: | Lipase B; p-NIPAM; Immobilization; Catalytic Activity; Thermoresponsibility |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-199499 |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 530 Physik 500 Naturwissenschaften und Mathematik > 540 Chemie |
| Fachbereich(e)/-gebiet(e): | 05 Fachbereich Physik 05 Fachbereich Physik > Institut für Physik Kondensierter Materie (IPKM) |
| Hinterlegungsdatum: | 06 Dez 2022 12:27 |
| Letzte Änderung: | 09 Dez 2022 08:56 |
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