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ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells

Eckert, Denise ; Rapp, Felicitas ; Tsedeke, Ayele T. ; Molendowska, Jessica ; Lehn, Robert ; Langhans, Markus ; Fournier, Claudia ; Rödel, Franz ; Hehlgans, Stephanie (2022)
ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells.
In: Cells, 2022, 11 (1)
doi: 10.26083/tuprints-00020277
Artikel, Zweitveröffentlichung, Verlagsversion

Kurzbeschreibung (Abstract)

Anti-inflammatory effects of low-dose irradiation often follow a non-linear dose–effect relationship. These characteristics were also described for the modulation of leukocyte adhesion to endothelial cells. Previous results further revealed a contribution of reactive oxygen species (ROS) and anti-oxidative factors to a reduced leukocyte adhesion. Here, we evaluated the expression of anti-oxidative enzymes and the transcription factor Nrf2 (Nuclear factor-erythroid-2-related factor 2), intracellular ROS content, and leukocyte adhesion in primary human microvascular endothelial cells (HMVEC) upon low-dose irradiation under physiological laminar shear stress or static conditions after irradiation with X-ray or Carbon (C)-ions (0–2 Gy). Laminar conditions contributed to increased mRNA expression of anti-oxidative factors and reduced ROS in HMVEC following a 0.1 Gy X-ray and 0.5 Gy C-ion exposure, corresponding to reduced leukocyte adhesion and expression of adhesion molecules. By contrast, mRNA expression of anti-oxidative markers and adhesion molecules, ROS, and leukocyte adhesion were not altered by irradiation under static conditions. In conclusion, irradiation of endothelial cells with low doses under physiological laminar conditions modulates the mRNA expression of key factors of the anti-oxidative system, the intracellular ROS contents of which contribute at least in part to leucocyte adhesion, dependent on the radiation source.

Typ des Eintrags: Artikel
Erschienen: 2022
Autor(en): Eckert, Denise ; Rapp, Felicitas ; Tsedeke, Ayele T. ; Molendowska, Jessica ; Lehn, Robert ; Langhans, Markus ; Fournier, Claudia ; Rödel, Franz ; Hehlgans, Stephanie
Art des Eintrags: Zweitveröffentlichung
Titel: ROS- and Radiation Source-Dependent Modulation of Leukocyte Adhesion to Primary Microvascular Endothelial Cells
Sprache: Englisch
Publikationsjahr: 2022
Publikationsdatum der Erstveröffentlichung: 2022
Verlag: MDPI
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Cells
Jahrgang/Volume einer Zeitschrift: 11
(Heft-)Nummer: 1
Kollation: 20 Seiten
DOI: 10.26083/tuprints-00020277
URL / URN: https://tuprints.ulb.tu-darmstadt.de/20277
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Herkunft: Zweitveröffentlichung DeepGreen
Kurzbeschreibung (Abstract):

Anti-inflammatory effects of low-dose irradiation often follow a non-linear dose–effect relationship. These characteristics were also described for the modulation of leukocyte adhesion to endothelial cells. Previous results further revealed a contribution of reactive oxygen species (ROS) and anti-oxidative factors to a reduced leukocyte adhesion. Here, we evaluated the expression of anti-oxidative enzymes and the transcription factor Nrf2 (Nuclear factor-erythroid-2-related factor 2), intracellular ROS content, and leukocyte adhesion in primary human microvascular endothelial cells (HMVEC) upon low-dose irradiation under physiological laminar shear stress or static conditions after irradiation with X-ray or Carbon (C)-ions (0–2 Gy). Laminar conditions contributed to increased mRNA expression of anti-oxidative factors and reduced ROS in HMVEC following a 0.1 Gy X-ray and 0.5 Gy C-ion exposure, corresponding to reduced leukocyte adhesion and expression of adhesion molecules. By contrast, mRNA expression of anti-oxidative markers and adhesion molecules, ROS, and leukocyte adhesion were not altered by irradiation under static conditions. In conclusion, irradiation of endothelial cells with low doses under physiological laminar conditions modulates the mRNA expression of key factors of the anti-oxidative system, the intracellular ROS contents of which contribute at least in part to leucocyte adhesion, dependent on the radiation source.

Freie Schlagworte: adhesion, endothelial cells, inflammation, leukocytes, low-dose irradiation, shear stress
Status: Verlagsversion
URN: urn:nbn:de:tuda-tuprints-202773
Sachgruppe der Dewey Dezimalklassifikatin (DDC): 500 Naturwissenschaften und Mathematik > 530 Physik
500 Naturwissenschaften und Mathematik > 540 Chemie
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin, Gesundheit
Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Membrane Dynamics
07 Fachbereich Chemie
07 Fachbereich Chemie > Ernst-Berl-Institut > Fachgebiet Makromolekulare Chemie
07 Fachbereich Chemie > Ernst-Berl-Institut > Fachgebiet Technische Chemie
Hinterlegungsdatum: 22 Apr 2022 12:17
Letzte Änderung: 25 Apr 2022 06:26
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