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The missing enzymatic link in syntrophic methane formation from fatty acids

Agne, Michael ; Estelmann, Sebastian ; Seelmann, Carola S. ; Kung, Johannes ; Wilkens, Dennis ; Koch, Hans-Georg ; Does, Chris van der ; Albers, Sonja V. ; Ballmoos, Christoph von ; Simon, Jörg ; Boll, Matthias (2021):
The missing enzymatic link in syntrophic methane formation from fatty acids.
In: Proceedings of the National Academy of Sciences of the United States of America, 118 (40), ISSN 1091-6490,
DOI: 10.1073/pnas.2111682118,
[Article]

Abstract

The microbial production of methane from organic matter is an essential process in the global carbon cycle and an important source of renewable energy. It involves the syntrophic interaction between methanogenic archaea and bacteria that convert primary fermentation products such as fatty acids to the methanogenic substrates acetate, H, CO, or formate. While the concept of syntrophic methane formation was developed half a century ago, the highly endergonic reduction of CO to methane by electrons derived from β-oxidation of saturated fatty acids has remained hypothetical. Here, we studied a previously noncharacterized membrane-bound oxidoreductase (EMO) from containing two heme cofactors and 8-methylmenaquinone as key redox components of the redox loop-driven reduction of CO by acyl-coenzyme A (CoA). Using solubilized EMO and proteoliposomes, we reconstituted the entire electron transfer chain from acyl-CoA to CO and identified the transfer from a high- to a low-potential heme with perfectly adjusted midpoint potentials as key steps in syntrophic fatty acid oxidation. The results close our gap of knowledge in the conversion of biomass into methane and identify EMOs as key players of β-oxidation in (methyl)menaquinone-containing organisms.

Item Type: Article
Erschienen: 2021
Creators: Agne, Michael ; Estelmann, Sebastian ; Seelmann, Carola S. ; Kung, Johannes ; Wilkens, Dennis ; Koch, Hans-Georg ; Does, Chris van der ; Albers, Sonja V. ; Ballmoos, Christoph von ; Simon, Jörg ; Boll, Matthias
Title: The missing enzymatic link in syntrophic methane formation from fatty acids
Language: English
Abstract:

The microbial production of methane from organic matter is an essential process in the global carbon cycle and an important source of renewable energy. It involves the syntrophic interaction between methanogenic archaea and bacteria that convert primary fermentation products such as fatty acids to the methanogenic substrates acetate, H, CO, or formate. While the concept of syntrophic methane formation was developed half a century ago, the highly endergonic reduction of CO to methane by electrons derived from β-oxidation of saturated fatty acids has remained hypothetical. Here, we studied a previously noncharacterized membrane-bound oxidoreductase (EMO) from containing two heme cofactors and 8-methylmenaquinone as key redox components of the redox loop-driven reduction of CO by acyl-coenzyme A (CoA). Using solubilized EMO and proteoliposomes, we reconstituted the entire electron transfer chain from acyl-CoA to CO and identified the transfer from a high- to a low-potential heme with perfectly adjusted midpoint potentials as key steps in syntrophic fatty acid oxidation. The results close our gap of knowledge in the conversion of biomass into methane and identify EMOs as key players of β-oxidation in (methyl)menaquinone-containing organisms.

Journal or Publication Title: Proceedings of the National Academy of Sciences of the United States of America
Journal volume: 118
Number: 40
Divisions: 10 Department of Biology
10 Department of Biology > Microbial Energy Conversion and Biotechnology
Date Deposited: 11 Oct 2021 06:31
DOI: 10.1073/pnas.2111682118
Additional Information:

Artikel-Nr.: e2111682118

Identification Number: pmid:34583996
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