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The missing enzymatic link in syntrophic methane formation from fatty acids

Agne, Michael ; Estelmann, Sebastian ; Seelmann, Carola S. ; Kung, Johannes ; Wilkens, Dennis ; Koch, Hans-Georg ; Does, Chris van der ; Albers, Sonja V. ; Ballmoos, Christoph von ; Simon, Jörg ; Boll, Matthias (2021)
The missing enzymatic link in syntrophic methane formation from fatty acids.
In: Proceedings of the National Academy of Sciences of the United States of America, 118 (40)
doi: 10.1073/pnas.2111682118
Artikel, Bibliographie

Kurzbeschreibung (Abstract)

The microbial production of methane from organic matter is an essential process in the global carbon cycle and an important source of renewable energy. It involves the syntrophic interaction between methanogenic archaea and bacteria that convert primary fermentation products such as fatty acids to the methanogenic substrates acetate, H, CO, or formate. While the concept of syntrophic methane formation was developed half a century ago, the highly endergonic reduction of CO to methane by electrons derived from β-oxidation of saturated fatty acids has remained hypothetical. Here, we studied a previously noncharacterized membrane-bound oxidoreductase (EMO) from containing two heme cofactors and 8-methylmenaquinone as key redox components of the redox loop-driven reduction of CO by acyl-coenzyme A (CoA). Using solubilized EMO and proteoliposomes, we reconstituted the entire electron transfer chain from acyl-CoA to CO and identified the transfer from a high- to a low-potential heme with perfectly adjusted midpoint potentials as key steps in syntrophic fatty acid oxidation. The results close our gap of knowledge in the conversion of biomass into methane and identify EMOs as key players of β-oxidation in (methyl)menaquinone-containing organisms.

Typ des Eintrags: Artikel
Erschienen: 2021
Autor(en): Agne, Michael ; Estelmann, Sebastian ; Seelmann, Carola S. ; Kung, Johannes ; Wilkens, Dennis ; Koch, Hans-Georg ; Does, Chris van der ; Albers, Sonja V. ; Ballmoos, Christoph von ; Simon, Jörg ; Boll, Matthias
Art des Eintrags: Bibliographie
Titel: The missing enzymatic link in syntrophic methane formation from fatty acids
Sprache: Englisch
Publikationsjahr: 5 Oktober 2021
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Proceedings of the National Academy of Sciences of the United States of America
Jahrgang/Volume einer Zeitschrift: 118
(Heft-)Nummer: 40
DOI: 10.1073/pnas.2111682118
Kurzbeschreibung (Abstract):

The microbial production of methane from organic matter is an essential process in the global carbon cycle and an important source of renewable energy. It involves the syntrophic interaction between methanogenic archaea and bacteria that convert primary fermentation products such as fatty acids to the methanogenic substrates acetate, H, CO, or formate. While the concept of syntrophic methane formation was developed half a century ago, the highly endergonic reduction of CO to methane by electrons derived from β-oxidation of saturated fatty acids has remained hypothetical. Here, we studied a previously noncharacterized membrane-bound oxidoreductase (EMO) from containing two heme cofactors and 8-methylmenaquinone as key redox components of the redox loop-driven reduction of CO by acyl-coenzyme A (CoA). Using solubilized EMO and proteoliposomes, we reconstituted the entire electron transfer chain from acyl-CoA to CO and identified the transfer from a high- to a low-potential heme with perfectly adjusted midpoint potentials as key steps in syntrophic fatty acid oxidation. The results close our gap of knowledge in the conversion of biomass into methane and identify EMOs as key players of β-oxidation in (methyl)menaquinone-containing organisms.

ID-Nummer: pmid:34583996
Zusätzliche Informationen:

Artikel-Nr.: e2111682118

Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Microbial Energy Conversion and Biotechnology
Hinterlegungsdatum: 11 Okt 2021 06:31
Letzte Änderung: 11 Okt 2021 06:32
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