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Sister chromatid exchanges occur in G₂-irradiated cells

Conrad, Sandro ; Künzel, Julia ; Löbrich, Markus (2021)
Sister chromatid exchanges occur in G₂-irradiated cells.
In: Cell Cycle, 10 (2)
doi: 10.26083/tuprints-00019036
Artikel, Zweitveröffentlichung, Verlagsversion

Kurzbeschreibung (Abstract)

DNA double-strand breaks (DSBs) are arguably the most important lesions induced by ionizing radiation (IR) since unrepaired or mis-repaired DSBs can lead to chromosomal aberrations and cell death. The two major pathways to repair IR-induced DSBs are non-homologous end-joining (NHEJ) and homologous recombination (HR). Perhaps surprisingly, NHEJ represents the predominant pathway in the G1 and G2 phases of the cell cycle, but HR also contributes and repairs a subset of IR-induced DSBs in G2. Following S-phase-dependent genotoxins, HR events give rise to sister chromatid exchanges (SCEs), which can be detected cytogenetically in mitosis. Here, we describe that HR occurring in G2-irradiated cells also generates SCEs in ~50% of HR events. Since HR of IR-induced DSBs in G2 is a slow process, SCE formation in G2-irradiated cells requires several hours. During this time, irradiated S-phase cells can also reach mitosis, which has contributed to the widely held belief that SCEs form only during S phase. We describe procedures to measure SCEs exclusively in G2-irradiated cells and provide evidence that following IR cells do not need to progress through S phase in order to form SCEs.

Typ des Eintrags: Artikel
Erschienen: 2021
Autor(en): Conrad, Sandro ; Künzel, Julia ; Löbrich, Markus
Art des Eintrags: Zweitveröffentlichung
Titel: Sister chromatid exchanges occur in G₂-irradiated cells
Sprache: Englisch
Publikationsjahr: 2021
Verlag: Taylor and Francis Group
Titel der Zeitschrift, Zeitung oder Schriftenreihe: Cell Cycle
Jahrgang/Volume einer Zeitschrift: 10
(Heft-)Nummer: 2
DOI: 10.26083/tuprints-00019036
URL / URN: https://tuprints.ulb.tu-darmstadt.de/19036
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Herkunft: Zweitveröffentlichungsservice
Kurzbeschreibung (Abstract):

DNA double-strand breaks (DSBs) are arguably the most important lesions induced by ionizing radiation (IR) since unrepaired or mis-repaired DSBs can lead to chromosomal aberrations and cell death. The two major pathways to repair IR-induced DSBs are non-homologous end-joining (NHEJ) and homologous recombination (HR). Perhaps surprisingly, NHEJ represents the predominant pathway in the G1 and G2 phases of the cell cycle, but HR also contributes and repairs a subset of IR-induced DSBs in G2. Following S-phase-dependent genotoxins, HR events give rise to sister chromatid exchanges (SCEs), which can be detected cytogenetically in mitosis. Here, we describe that HR occurring in G2-irradiated cells also generates SCEs in ~50% of HR events. Since HR of IR-induced DSBs in G2 is a slow process, SCE formation in G2-irradiated cells requires several hours. During this time, irradiated S-phase cells can also reach mitosis, which has contributed to the widely held belief that SCEs form only during S phase. We describe procedures to measure SCEs exclusively in G2-irradiated cells and provide evidence that following IR cells do not need to progress through S phase in order to form SCEs.

Status: Verlagsversion
URN: urn:nbn:de:tuda-tuprints-190369
Sachgruppe der Dewey Dezimalklassifikatin (DDC): 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie
Fachbereich(e)/-gebiet(e): 10 Fachbereich Biologie
10 Fachbereich Biologie > Radiation Biology and DNA Repair
Hinterlegungsdatum: 08 Sep 2021 12:09
Letzte Änderung: 13 Sep 2021 06:26
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