Engel, Anja J. ; Kithil, Marina ; Langhans, Markus ; Rauh, Oliver ; Cartolano, Matea ; Etten, James L. van ; Moroni, Anna ; Thiel, Gerhard (2021)
Codon Bias Can Determine Sorting of a Potassium Channel Protein.
In: Cells, 2021, 10 (5)
doi: 10.26083/tuprints-00019352
Artikel, Zweitveröffentlichung, Verlagsversion
Kurzbeschreibung (Abstract)
Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, translation and folding. Here, we use two similar prototype K+ channels as model systems to examine whether codon choice has an impact on protein sorting. By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. The data establish that a gene with either rare or frequent codons serves, together with a cell-state-dependent decoding mechanism, as a secondary code for sorting intracellular membrane proteins.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2021 |
| Autor(en): | Engel, Anja J. ; Kithil, Marina ; Langhans, Markus ; Rauh, Oliver ; Cartolano, Matea ; Etten, James L. van ; Moroni, Anna ; Thiel, Gerhard |
| Art des Eintrags: | Zweitveröffentlichung |
| Titel: | Codon Bias Can Determine Sorting of a Potassium Channel Protein |
| Sprache: | Englisch |
| Publikationsjahr: | 2021 |
| Publikationsdatum der Erstveröffentlichung: | 2021 |
| Verlag: | MDPI |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Cells |
| Jahrgang/Volume einer Zeitschrift: | 10 |
| (Heft-)Nummer: | 5 |
| Kollation: | 17 Seiten |
| DOI: | 10.26083/tuprints-00019352 |
| URL / URN: | https://tuprints.ulb.tu-darmstadt.de/19352 |
| Zugehörige Links: | |
| Herkunft: | Zweitveröffentlichung aus gefördertem Golden Open Access |
| Kurzbeschreibung (Abstract): | Due to the redundancy of the genetic code most amino acids are encoded by multiple synonymous codons. It has been proposed that a biased frequency of synonymous codons can affect the function of proteins by modulating distinct steps in transcription, translation and folding. Here, we use two similar prototype K+ channels as model systems to examine whether codon choice has an impact on protein sorting. By monitoring transient expression of GFP-tagged channels in mammalian cells, we find that one of the two channels is sorted in a codon and cell cycle-dependent manner either to mitochondria or the secretory pathway. The data establish that a gene with either rare or frequent codons serves, together with a cell-state-dependent decoding mechanism, as a secondary code for sorting intracellular membrane proteins. |
| Status: | Verlagsversion |
| URN: | urn:nbn:de:tuda-tuprints-193522 |
| Zusätzliche Informationen: | Keywords: codon usage; effect of synonymous codon exchange; membrane protein sorting; dual sorting |
| Sachgruppe der Dewey Dezimalklassifikatin (DDC): | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften, Biologie |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Plant Membrane Biophyscis (am 20.12.23 umbenannt in Biologie der Algen und Protozoen) |
| Hinterlegungsdatum: | 25 Aug 2021 12:25 |
| Letzte Änderung: | 22 Nov 2021 10:19 |
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