Gnan, Stefano ; Flyamer, Ilya M. ; Klein, Kyle N. ; Castelli, Eleonora ; Rapp, Alexander ; Maiser, Andreas ; Chen, Naiming ; Weber, Patrick ; Enervald, Elin ; Cardoso, M. Cristina ; Bickmore, Wendy A. ; Gilbert, David M. ; Buonomo, Sara C. B. (2021)
Nuclear organisation and replication timing are coupled through RIF1-PP1 interaction.
In: Nature communications, 12 (1)
doi: 10.1038/s41467-021-22899-2
Artikel, Bibliographie
Kurzbeschreibung (Abstract)
Three-dimensional genome organisation and replication timing are known to be correlated, however, it remains unknown whether nuclear architecture overall plays an instructive role in the replication-timing programme and, if so, how. Here we demonstrate that RIF1 is a molecular hub that co-regulates both processes. Both nuclear organisation and replication timing depend upon the interaction between RIF1 and PP1. However, whereas nuclear architecture requires the full complement of RIF1 and its interaction with PP1, replication timing is not sensitive to RIF1 dosage. The role of RIF1 in replication timing also extends beyond its interaction with PP1. Availing of this separation-of-function approach, we have therefore identified in RIF1 dual function the molecular bases of the co-dependency of the replication-timing programme and nuclear architecture.
| Typ des Eintrags: | Artikel |
|---|---|
| Erschienen: | 2021 |
| Autor(en): | Gnan, Stefano ; Flyamer, Ilya M. ; Klein, Kyle N. ; Castelli, Eleonora ; Rapp, Alexander ; Maiser, Andreas ; Chen, Naiming ; Weber, Patrick ; Enervald, Elin ; Cardoso, M. Cristina ; Bickmore, Wendy A. ; Gilbert, David M. ; Buonomo, Sara C. B. |
| Art des Eintrags: | Bibliographie |
| Titel: | Nuclear organisation and replication timing are coupled through RIF1-PP1 interaction. |
| Sprache: | Englisch |
| Publikationsjahr: | 18 Mai 2021 |
| Titel der Zeitschrift, Zeitung oder Schriftenreihe: | Nature communications |
| Jahrgang/Volume einer Zeitschrift: | 12 |
| (Heft-)Nummer: | 1 |
| DOI: | 10.1038/s41467-021-22899-2 |
| Kurzbeschreibung (Abstract): | Three-dimensional genome organisation and replication timing are known to be correlated, however, it remains unknown whether nuclear architecture overall plays an instructive role in the replication-timing programme and, if so, how. Here we demonstrate that RIF1 is a molecular hub that co-regulates both processes. Both nuclear organisation and replication timing depend upon the interaction between RIF1 and PP1. However, whereas nuclear architecture requires the full complement of RIF1 and its interaction with PP1, replication timing is not sensitive to RIF1 dosage. The role of RIF1 in replication timing also extends beyond its interaction with PP1. Availing of this separation-of-function approach, we have therefore identified in RIF1 dual function the molecular bases of the co-dependency of the replication-timing programme and nuclear architecture. |
| ID-Nummer: | pmid:34006872 |
| Fachbereich(e)/-gebiet(e): | 10 Fachbereich Biologie 10 Fachbereich Biologie > Cell Biology and Epigenetics |
| Hinterlegungsdatum: | 25 Mai 2021 06:11 |
| Letzte Änderung: | 25 Mai 2021 06:11 |
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